Articles
MICROPROPAGATION OF SOME MEDICINAL PLANTS OF ARAVALLIS IN RAJASTHAN – CHEAPER AND BETTER OPTIONS
Article number
1030_13
Pages
109 – 117
Language
English
Abstract
Recent studies in our laboratory included evaluation of innovative approaches such as photoautotrophic cultivation of tissue culture plants under CO2 enriched environment, liquid culture system, culture vessel ventilation and biopriming during hardening and acclimatization of three medicinally important plants viz; Celastrus paniculatus (Mal Kangni), Chlorophytum borivilianum (Safed Musli) and Boswellia serrata (Indian Olibanum). The results have shown that cultures grew better in the environment enriched with CO2 and on the media devoid of sucrose.
Liquid cultures supported by borosilicate glass beads promoted growth with no apparent adverse effects.
Growing cultures under proper ventilation through the use of indigenously designed caps has proved to be of great advantage in the development of healthy cultures.
Experiments with inoculation of Piriformospora indica, a root endophytic fungus, for root colonization during hardening and acclimatization have not only shown enhanced growth but also improvement in the rate of survival of tissue culture raised plantlets.
Liquid cultures supported by borosilicate glass beads promoted growth with no apparent adverse effects.
Growing cultures under proper ventilation through the use of indigenously designed caps has proved to be of great advantage in the development of healthy cultures.
Experiments with inoculation of Piriformospora indica, a root endophytic fungus, for root colonization during hardening and acclimatization have not only shown enhanced growth but also improvement in the rate of survival of tissue culture raised plantlets.
Authors
S.D. Purohit, N. Habibi
Keywords
liquid culture system, photoautotrophic cultivation, vented caps, biopriming, medicinal plants
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