Articles
ANALYSIS OF THE TIME-DEPENDENT PROTEIN CHANGES IN WOUNDED APPLES (‘GOLDEN DELICIOUS’)
Article number
1053_17
Pages
171 – 176
Language
English
Abstract
Malus × domestica Borkh. Golden Delicious is one of the most important apple cultivars worldwide.
However, if damaged, the fruit becomes susceptible to opportunistic infection by postharvest phytopathogens such as Botrytis cinerea (gray mould) and Penicillium expansum (blue mould) which annually lead to large economic losses worldwide.
Therefore, a proteomic study of the fruit response to wounding would contribute to better understanding of the physiological mechanisms underlying fruit stress response.
In this study, we provide the first systematic description of time-dependent protein changes in wounded Golden Delicious apples, using 2D-PAGE and MS for protein identification.
Publicly available protein and EST databases were used for protein identification and annotation.
Proteins that did not change in abundance after wounding were mainly categorized in the biological process of metabolism, response to stress, and oxidation-reduction processes, whereas proteins with changes in abundance after wounding were mainly categorized in response to stress. We suggest that fruits react to mechanical damage by synthesizing a broad range of PR proteins.
Increasing our knowledge of apple fruit proteins in wounded fruit tissues will be useful in developing new strategies to minimize postharvest losses.
However, if damaged, the fruit becomes susceptible to opportunistic infection by postharvest phytopathogens such as Botrytis cinerea (gray mould) and Penicillium expansum (blue mould) which annually lead to large economic losses worldwide.
Therefore, a proteomic study of the fruit response to wounding would contribute to better understanding of the physiological mechanisms underlying fruit stress response.
In this study, we provide the first systematic description of time-dependent protein changes in wounded Golden Delicious apples, using 2D-PAGE and MS for protein identification.
Publicly available protein and EST databases were used for protein identification and annotation.
Proteins that did not change in abundance after wounding were mainly categorized in the biological process of metabolism, response to stress, and oxidation-reduction processes, whereas proteins with changes in abundance after wounding were mainly categorized in response to stress. We suggest that fruits react to mechanical damage by synthesizing a broad range of PR proteins.
Increasing our knowledge of apple fruit proteins in wounded fruit tissues will be useful in developing new strategies to minimize postharvest losses.
Authors
G. Buron-Moles, I. Viñas, N. Teixidó, J. Usall, R. Torres, M. Davey, F. Amoako-Andoh, W. Keulemans
Keywords
Malus × domestica, abiotic stress, mechanical damage, phenol-based method, MALDI-TOF, defence response
Online Articles (30)