Articles
THE USE OF META-TOPOLIN AS AN ALTERNATIVE CYTOKININ IN THE TISSUE CULTURE OF EUCALYPTUS SPECIES©
Article number
1055_4
Pages
25 – 28
Language
English
Abstract
Meta-topolin (mT) is a relatively new cytokinin isolated from poplar leaves in 1975 and is closely related to 6-benzyladenine (BA). Research on the use of mT in tissue culture has been conducted on several species, including Hypericum, citrus rootstock, Aloe, banana (Musa acuminate), pineapple (Ananas comosus), and Barleria. 6-Benzyladenine (BA) is the most widely used cytokinin in the regeneration stage of the tissue culture of most plant species, because of its availability and price, but it has a few drawbacks which include causing the hyperhydricity (vitrification) of shoots and it can have a negative effect on rooting.
In light of this a series of trials were initiated to determine the effect of mT on the regeneration, hyperhydricity, and rooting of Eucalyptus species.
In the initial trial various concentrations of mT (ranging from 1.2 to 14.5 mgL-1) were tested with the resulting shoot growth compact and stunted.
In a follow-up trial, a mT concentration of 0.2 mgL-1 was found to produce shoots that were less vitrified and that resulted in better in vitro rooting.
Further trials on rooting of other eucalypt species are in progress to determine the benefits of mT.
In light of this a series of trials were initiated to determine the effect of mT on the regeneration, hyperhydricity, and rooting of Eucalyptus species.
In the initial trial various concentrations of mT (ranging from 1.2 to 14.5 mgL-1) were tested with the resulting shoot growth compact and stunted.
In a follow-up trial, a mT concentration of 0.2 mgL-1 was found to produce shoots that were less vitrified and that resulted in better in vitro rooting.
Further trials on rooting of other eucalypt species are in progress to determine the benefits of mT.
Authors
A. van der Westhuizen
Keywords
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