Home > VI International Symposium on Production and Establishment of Micropropagated Plants > Legume biotechnology: towards development of a genetic transformation system for commercial cultivars of soybean (Glycine max L.)

Articles

Legume biotechnology: towards development of a genetic transformation system for commercial cultivars of soybean (Glycine max L.)

Article number

1155_67

Pages

455 – 460

Language

English

Abstract

Legume plants are a major source of proteins for human and animals.
Legumes have the unique capacity to fix atmospheric nitrogen.
Soybean is a major legume crop providing oilseed, used for various purposes.
Soybean genome sequence has been recently made available.
Advances in genomics can be fully utilized with the development of genetic transformation systems for commercial cultivars.
To this end, we aim to develop an efficient method for genetic transformation of commercial cultivars of soybean.
Therefore, we evaluated different seedling explants, cultivars and selective marker genes.
Our result indicated the importance of using a combination of antibiotics and explant type during Agrobacterium-mediated transformation.
Also, the use of Basta to select transgenic cells and shoots is highlighted.

Publication

VI International Symposium on Production and Establishment of Micropropagated Plants

Authors

S.-I. Lee, M.B. Singh, P.L. Bhalla

Keywords

Agrobacterium, glufosinate, oilseed crops, seed explants, selectable marker

Full text

https://doi.org/10.17660/ActaHortic.2017.1155.67

Online Articles (92)

1. The role of stress on unravelling of somatic embryogenesis competence

S.J. Ochatt

2. Factors affecting the axillary and adventitious shoot formation in woody plants in vitro

I.A. Iliev

3. Trends in culture medium nitrogen requirements for in vitro shoot growth of diverse pear germplasm

S. Wada | B.M. Reed

4. Castanea spp. hybrid clones in vitro conservation: synthetic seeds vs. slow growth storage

5. A multi-year study on the regenerative potential of several Vitis genotypes

I. Gribaudo | G. Gambino | P. Boccacci | I. Perrone | D. Cuozzo

6. Temporary immersion bioreactors for large scale Globba micropropagation

N. Topoonyanont | P. Pumisutapon | S. Klayraung | P. Poonnoy

7. Encapsulation of lateral buds of Hancornia speciosa

8. Encapsulation of black mulberry microcuttings: studies on capsules and synthetic seeds

9. Comparison of two protocols for somatic embryo induction in a Sulawesi elite Theobroma cacao L. clone

M.U. Bustami | S. Werbrouck

10. Are we able to modulate the response of somatic embryos of pines to drought stress?

O. García-Mendiguren | I.A. Montalbán | T. Goicoa | M.D. Ugarte | P. Moncaleán

11. Micropropagation of mature Juglans hybrids

A. Meier-Dinkel | I. Wenzlitschke

12. Micropropagation of common ash clones resistant to fungus Hymenoscyphus fraxineus

J. ¿edivá | L. Havrdová | P. Mar¿ík

13. In vitro conservation of fruit trees by slow growth storage

A. Arbeloa | J.A. Marín | P. Andreu | E. García | P. Lorente

14. An efficient in vitro propagation for faba bean (Vicia faba L.) ecotypes

C. Ruta | A. Campanelli | A. Tagarelli | G. De Mastro | C. Vancini

15. Gentian ‘Little Pinkie’ and the production of explants as influenced by ethephon and growth duration in vitro

M. Keshavarzi | J.A. Heyes | D.J. Woolley | K.A. Funnell

16. Efficient Lilium longiflorum plantlet regeneration from transgenic callus culture lines after ten years of sub-culture

A. Lipsky | A. Cohen | Z. Machbash | S. Yariv | I. Yedidia

17. The improvement of Iris pallida propagation by somatic embryogenesis

18. Development, maturation and conversion of somatic embryos of Actaea racemosa L.

I. Pinker | M. Wernicke

19. In vitro culture to improve breeding activities in Rosa hybrida

20. Establishment and in vitro multiplication of Calophyllum brasiliensis

21. Development of tissue culture system for pear cultivars

J. Sedlak | F. Paprstein

22. Identification of a cytokinin treatment for in vitro propagation of two sweet cherry cultivars in preparation of a sanitation protocol

F. Paprstein | J. Sedlak

23. In vitro propagation of Italian cultivars of Vitis vinifera and evaluation of genetic stability by SSRs markers

E. Gatti | S.A. Imazio | E. Sgarbi

24. Effect of 6-benzylaminopurine and coconut water on shoot multiplication of Heliconia chartacea ‘Sexy Pink’

M.D.M. Raizer | J.H. Iriarte-Martel | M.T. Lopes | R.C. Quisen

25. In vitro organogenesis in Handroanthus serratifolius

26. The effects of meta-Topolin and benzyladenine on in vitro organogenesis from epicotyl cuttings of Troyer citrange (Citrus sinensis [L.] Osbeck × Poncirus trifoliata [L.] Raf.)

B. Chiancone | L. Martorana | V. Gianguzzi | M.A. Germanà

27. Optimization of in vitro growth medium for a wild Kazakhstan apricot, Prunus armeniaca

I.Y. Kovalchuk | Z.R. Mukhitdinova | T.T. Turdiyev | G.A. Madiyeva | B.M. Reed

28. Tissue culture techniques as a tool to select snapdragon mutants with differential NaCl sensitivity

29. In vitro callogenesis and screening of local varieties of Vicia faba L. to salt tolerance

A.H. Al Maamory | A. Tarang | L. Chalak

30. Moderate-abiotic stress increase in vitro tuberization and microtuber growth of potato

P. Pumisutapon | N. Topoonyanont

31. Improving in vitro mass proliferation of carob (Ceratonia siliqua L.) from seedling apices by temporary immersion systems

32. Temporary immersion system: an efficient technique to improve the Plumeria rubra L. scale-up

M. Airò | M.M. Mammano | G. Giardina | A. Giovino

33. Plant tissue culture contamination: challenges and opportunities

E.B. Herman

34. Volatile indicators of contamination in tissue cultures

S. Werbrouck | P. Verholle | H. Van Langenhove

35. Effect of carbon nanotubes in micropropagation of GF677 (Prunus amygdalus × Prunus persica) rootstock

H. Sarikhani | H. Ghorbanizad | M. Gholami

36. Effect of BAP on growth, amino acid content and lipid fatty acid composition of curly birch in vitro shoots

L. Vetchinnikova | T. Kuznetsova | A. Titov

37. The effects of low-level laser irradiation on growth of microshoots of blackberry

G.R. Verulidze | D.D. Surmanidze

38. Molecular markers, clonal fidelity and antioxidant properties of micropropagated horticultural crops

S.C. Debnath

39. Hyperhydricity: underlying mechanisms

G.-J.M. de Klerk | N. van den Dries | F.A. Krens

40. Effect of plant growth regulators, light intensity and LED on growth and volatile compound of Hyptis suaveolens (L.) Poit in vitro plantlets

41. Effects of Vitrofural, BAP and meta-Topolin in the in vitro culture of Dendrocalamus asper

T.S. Ornellas | D. Werner | D.F. Holderbaum | R.F. Scherer | M.P. Guerra

42. In vitro propagation of Habenaria hybrids

C. Sotthikul | C. Kaewpoowat | N. Saimoon

43. In vitro propagation as a tool to enhance the use of native ornamentals in archaeological sites of Greece

M. Papafotiou | A.N. Martini | G. Vlachou

44. Effect of chemical and physical factors in in vitro propagation and volatile fraction analysis of Aloysia triphylla (L’Herit) Britton

45. Studies on in vitro propagation of Anthyllis barba-jovis L.

G. Vlachou | M. Papafotiou | K.F. Bertsouklis

46. Propagation of pistachio applying in vitro culture techniques

J.A. Marín | E. García | P. Lorente | A. Arbeloa | P. Andreu

47. In vitro propagation of Lippia citriodora

M. Papafotiou | N. Tsalouchos | A. Papagianni | K.F. Bertsouklis

48. In vitro culture of clonal rootstocks of apple for their commercial exploitation

M. Modgil | M. Thakur

49. Effects of cytokinin treatment of nodal cuts and petioles on shoot regeneration of Campanula portenschlagiana ‘Blue Ocean’

Z. Ghayoor Karimiani | H. Mibus | M. Serek

50. Effect of auxin and polyamines on protein contents and peroxidase activity during in vitro culture of taro

51. Meta-topolin improves lateral bud proliferation in micropropagation of Ginkgo biloba L.

L. Nacheva | P. Gercheva | V. Ivanova | O. Ibrahim

52. In vitro regeneration, proliferation and growth of strawberry under different light treatments

F.H. Mohamed | G.F. Omar | M.A. Ismail

53. In vitro propagation of red raspberry under light-emitting diodes (LEDs)

P. Poncetta | D. Ioratti | I. Mignani | L. Giongo

54. Effectiveness of LED and fluorescent light on in vitro shoot proliferation of Staphylea pinnata

B. Szewczyk-Taranek | B. Pawłowska | B. Prokopiuk | M. Żupnik

55. Effects of the natural cytokinin meta-Topolin on in vitro shoot proliferation and acclimatization of Prunus spp.

S. Monticelli | A. Gentile | A. Frattarelli | E. Caboni

56. Influence of ozone treatments on in vitro propagation of Lilium in bioreactor

M. Cardarelli | C.M. Cardona Suárez

57. Effect of weak magnetic fields on the in vitro propagation of Genista aetnensis (Raf. Ex Biv.) Dc.

58. Influence of culture vessels on mesophyll protoplast yield of Pelargonium ssp.

E. Klocke | K. Weinzierl

59. Effect of nitrogen, phosphorus and calcium on in vitro growth and development of Aechmea blanchetiana

A.R. Tavares | A.N. Gonçalves | R.C.S. Ribeiro | T. Jocys | S. Kanashiro

60. In vitro culture of Aloe arborescens shoots using light-emitting diodes

M. Cardarelli | V. Pizzinelli

61. Asymbiotic seed germination of hand-pollinated terrestrial orchids

J. Calevo | A. Giovannini | L. Cornara | S. Peccenini

62. Meristem tip culture of Dendrobium orchid for boosting efficiency of hygienic large scale micropropagation

N. Kunagorn | C. Roopkam | P. Aumroong | N. Anukul

63. The use of in vitro chemotherapy in grapevine virus elimination

I.-C. Guţă | E.-C. Buciumeanu | C.M. Topală | L.D. Tătaru

64. Virus elimination in peach using chemotherapy

M. Kudělková | R. Pavelková | E. Ondru¿iková

65. Diversity and control of bacterial contamination of plants propagated in temporary immersion bioreactor system

S. Klayraung | P. Niamsup | P. Poonnoy | N. Topoonyanont

66. Features of canna regeneration in vitro and plantlets adaptation in vivo

I.V. Mitrofanova | A.Sh. Tevfik | O.V. Mitrofanova | V.A. Brailko | N.P. Lesnikova-Sedoshenko

67. Legume biotechnology: towards development of a genetic transformation system for commercial cultivars of soybean (Glycine max L.)

S.-I. Lee | M.B. Singh | P.L. Bhalla

68. Reduction of the subculture frequency in three Mediterranean species during micropropagation

C. Mascarello | E. Sacco | D. Di Silvestro | M. Pamato | B. Ruffoni

69. Effects of media and hormones on the adventitious shoot regeneration and development of Manchurian ash

70. Acclimation ex vitro: how to improve the root system

D. Neri | E.M. Lodolini | M. Bastianelli

71. Commercial use of endophytes in micropropagation

C. Schneider | I Hutter | M. Döring

72. Steering stomatal dynamics by chemicals during acclimatization of Spathiphyllum

E. Dhooghe | R. Dierck | J. Van Huylenbroeck

73. New cytokinin-like compounds as a tool to improve rooting and establishment of micropropagated plantlets

A.O. Aremu | K. Dole¿al | J. Van Staden

74. Adventitious root formation in Eucalyptus: the role of phytohormones

M. Nakhooda | M.P. Watt

75. Burkholderia phytofirmans PsJN promotes in vitro rooting and acclimatization of Helleborus

T. Orlikowska | K. Nowak | L. Ogórek

76. Rooting response under LED systems in Rosa canina in vitro cultures

B. Pawłowska | B. Szewczyk-Taranek | E. Dziedzic | M. Żupnik

77. Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro

P.N. Tuan | A. Meier-Dinkel | A.M. Höltken | I. Wenzlitschke | T. Winkelmann

78. In vitro rooting of micropropagated shoots from Myrtus communis Linn: influence of activated charcoal and indole-3-butyric acid (IBA)

Y. Aka Kaçar | Ö. Şimşek | B. Biçen | B. Dal

79. In vitro rooting of Acca sellowiana microshoots

80. Rooting and acclimatization of micropropagated Hypericum perforatum L. native to Sicily

81. Arbuscular mycorrhizal fungi and micropropagation of Ranunculus asiaticus L.: a useful alliance?

R. Borriello | D. Maccario | S. Viglione | V. Bianciotto | M. Beruto

82. Effect of a non-mycorrhizal endophyte isolated from Mentha piperita on in vitro culture of Ocimum basilicum cuttings

F. Dovana | A. Fusconi | G. Berta | W. Camusso | M. Mucciarelli

83. Volatile compounds of ex vitro and wild plantlets of Artemisia umbelliformis subsp. eriantha (Apennines’ genepì)

P. Fasciani | G. Marcozzi | S. Reale | L. Pace

84. The potential of bioreactor technology for large-scale plant micropropagation

R. Ibrahim

85. Biopharming Carica papaya compounds with antibacterial efficacy

N. Jamieson | R. Drew | I. Cock

86. Usefulness of in vitro micropropagation for establishing plantations of self-rooted Hevea brasiliensis industrial clones

A. Masson

87. Establishment of in vitro plants selected from heavy metal contaminated soils for further phytoremediation use

L. Pistelli | F. D¿Angiolillo | M. Bortolazzo | E. Morelli | B. Basso

88. Establishment of vitroplants from female flowers of Aerva javanica (Burm. F) Juss ex Schult.

D. Chabane | F. Mouhoub

89. Micropropagation of old cultivars of scented cut roses

A. Giovannini | L. De Benedetti | F. Crippa | A. Mercuri

90. Micropropagation of rare and threatened medicinal plant species of South Africa – for propagation and preservation: an overview

V. Reddy

91. In vitro propagation of Cotula bipinnata Thunb. and Chironia linoides L., South African ornamental species suitable for cultivation in Mediterranean area

E. Sacco | C. Mascarello | M. Pamato | D. Di Silvestro | B. Ruffoni

92. Rosmarinus officinalis L.: micropropagation and callus induction for cell biomass development