Articles
Bulblet regeneration in vitro from bulb-scales of Fritillaria thunbergii
Article number
1171_4
Pages
25 – 30
Language
English
Abstract
Fritillaria thunbergii (Liliaceae), commonly known in Chinese as zhebei, is a perennial plant native to China with important medicinal and ornamental value.
Wild populations are at a risk of rapid eradication, and are now very difficult to find.
In order to protect wild resources and to set up an efficient system of rapid propagation, sections of bulb-scales from the vegetative period were used to regenerate bulblets in vitro.
Surface-disinfected sections were cultured in Murashige and Skoog (MS) medium containing 35 g L-1 sucrose and supplemented with different types, concentrations and combinations of plant growth regulators [α-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KT)]. Bulblets were successfully induced from scale sections after 12 weeks of culture via two pathways.
In the first pathway, callus was induced within 5 weeks from scale sections on solid MS medium that was supplemented with 0.5 mg L-1 BA and 2.0 mg L-1 NAA. After placing callus at low temperature (4°C) for 3 weeks, bulblets developed on the same medium within 4 weeks (11.3 bulblets explant-1). In the second (less effective) pathway, bulblets could be induced directly from scale sections on solid MS medium supplemented with 2.0 mg L-1 BA and 0.5 mg L-1 NAA, or 1.0 mg L-1 BA and 0.1 mg L-1 2,4-D, resulting in 8.2 and 7.8 bulblets explant-1, respectively.
This is a simple but effective protocol for the mass propagation of F. thunbergii.
Wild populations are at a risk of rapid eradication, and are now very difficult to find.
In order to protect wild resources and to set up an efficient system of rapid propagation, sections of bulb-scales from the vegetative period were used to regenerate bulblets in vitro.
Surface-disinfected sections were cultured in Murashige and Skoog (MS) medium containing 35 g L-1 sucrose and supplemented with different types, concentrations and combinations of plant growth regulators [α-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KT)]. Bulblets were successfully induced from scale sections after 12 weeks of culture via two pathways.
In the first pathway, callus was induced within 5 weeks from scale sections on solid MS medium that was supplemented with 0.5 mg L-1 BA and 2.0 mg L-1 NAA. After placing callus at low temperature (4°C) for 3 weeks, bulblets developed on the same medium within 4 weeks (11.3 bulblets explant-1). In the second (less effective) pathway, bulblets could be induced directly from scale sections on solid MS medium supplemented with 2.0 mg L-1 BA and 0.5 mg L-1 NAA, or 1.0 mg L-1 BA and 0.1 mg L-1 2,4-D, resulting in 8.2 and 7.8 bulblets explant-1, respectively.
This is a simple but effective protocol for the mass propagation of F. thunbergii.
Authors
L.H. Hao, J.A. Teixeira da Silva, X.N. Yu
Keywords
fritillaries, traditional Chinese medicine, tissue culture, organ culture, micropropagation, conservation
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