Articles
Etiological study of viruses associated with yellow mosaic disease in jasmine
Article number
1193_7
Pages
47 – 54
Language
English
Abstract
Jasmine (Jasminum sambac) is widely grown as an ornamental plant and for extraction of essential oil in Taiwan.
In 2003, a potyvirus, designated Jasmine virus T (JaVT), was isolated from jasmine exhibiting severe yellow mosaic symptoms.
However, due to jasmine’s vegetative propagation nature, confirmed virus-free jasmine plants were unavailable for inoculation tests.
Determining whether JaVT could individually induce yellow mosaic symptom was therefore not testable.
In 2010, another field survey was conducted and found that, in addition to plants showing a yellow mosaic symptom, there were also jasmine exhibiting mild mosaic, mottling symptoms or even being nearly symptomless.
Interestingly, JaVT could be detected from all of the jasmine plants exhibiting these diverse symptoms, suggesting JaVT might not be the only virus inducing the yellow mosaic disease.
In 2012, a Carlavirus was detected from yellow mosaic jasmine plants by RT-PCR using a degenerate Carlavirus-specific primer pair.
Sequence analysis of these amplicons demonstrated the presence of a new species of Carlavirus, which was named Jasmine virus C (JaVC). Virus-specific ELISA and RT-PCR analysis confirmed that JaVC could only be detected in yellow mosaic jasmine, but not in those plants showing mild symptoms.
Taken together, these results suggest that the yellow mosaic symptom in jasmine was induced by a mixed infection of JaVT and JaVC.
In 2003, a potyvirus, designated Jasmine virus T (JaVT), was isolated from jasmine exhibiting severe yellow mosaic symptoms.
However, due to jasmine’s vegetative propagation nature, confirmed virus-free jasmine plants were unavailable for inoculation tests.
Determining whether JaVT could individually induce yellow mosaic symptom was therefore not testable.
In 2010, another field survey was conducted and found that, in addition to plants showing a yellow mosaic symptom, there were also jasmine exhibiting mild mosaic, mottling symptoms or even being nearly symptomless.
Interestingly, JaVT could be detected from all of the jasmine plants exhibiting these diverse symptoms, suggesting JaVT might not be the only virus inducing the yellow mosaic disease.
In 2012, a Carlavirus was detected from yellow mosaic jasmine plants by RT-PCR using a degenerate Carlavirus-specific primer pair.
Sequence analysis of these amplicons demonstrated the presence of a new species of Carlavirus, which was named Jasmine virus C (JaVC). Virus-specific ELISA and RT-PCR analysis confirmed that JaVC could only be detected in yellow mosaic jasmine, but not in those plants showing mild symptoms.
Taken together, these results suggest that the yellow mosaic symptom in jasmine was induced by a mixed infection of JaVT and JaVC.
Authors
C.A. Chang, Y.C. Lin, Y.Y. Lin, S.M. Wang
Keywords
Potyvirus, Jasmine, Jasminum sambac, JaVT, Carlavirus, JaVC, etiology, mixed infection
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