Articles
Molecular cloning, expression and phylogenetic analysis of a dormancy related DAM gene in almond (Prunus dulcis)
Article number
1229_10
Pages
61 – 64
Language
English
Abstract
Flowering time in almond is a character of great interest in the development of new extra-late cultivars adapted to cold areas.
Late flowering is related to a long dormancy period due to high chilling requirements of the cultivar.
It is considered a quantitative and highly heritable character but a dominant gene (late bloom, Lb) was also described.
A major QTL (quantitative trait loci) in the linkage group (LG) 4 was associated with the Lb, together with other three minor QTLs in LG1 and LG7. On the other hand, DAM (dormancy-associated MADS-Box) genes located in LG1 have been largely described as a gene family involved in bud dormancy.
In order to study the LG1 region involved in flowering time and dormancy regulation, a DAM gene was characterized in almond.
The full-length cDNA clone, designated PdDAM6, was isolated using 3′-RACE strategy in two almond cultivars with different chilling requirements and flowering time: ‘Desmayo Largueta’ (extra-early flowering) and ‘Tardona’ (ultra-late flowering). A phylogenetic analysis of the sequences was also performed with homologous sequences from other Prunus species.
Late flowering is related to a long dormancy period due to high chilling requirements of the cultivar.
It is considered a quantitative and highly heritable character but a dominant gene (late bloom, Lb) was also described.
A major QTL (quantitative trait loci) in the linkage group (LG) 4 was associated with the Lb, together with other three minor QTLs in LG1 and LG7. On the other hand, DAM (dormancy-associated MADS-Box) genes located in LG1 have been largely described as a gene family involved in bud dormancy.
In order to study the LG1 region involved in flowering time and dormancy regulation, a DAM gene was characterized in almond.
The full-length cDNA clone, designated PdDAM6, was isolated using 3′-RACE strategy in two almond cultivars with different chilling requirements and flowering time: ‘Desmayo Largueta’ (extra-early flowering) and ‘Tardona’ (ultra-late flowering). A phylogenetic analysis of the sequences was also performed with homologous sequences from other Prunus species.
Authors
A.S. Prudencio, F. Dicenta, P. Martínez-Gómez
Keywords
flowering, Prunus dulcis, dormancy, qPCR, transcription factors, breeding
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