Articles
Genetic profiling of wild accessions of papaya (Carica papaya L.) collected in Yucatan state by using amplified fragment length polymorphism (AFLP) markers
Article number
1250_11
Pages
69 – 76
Language
English
Abstract
The Yucatan Peninsula, together with Central America, has been considered the center of origin of papaya (Carica papaya L.). The wide geographic distribution of wild accessions and the lack of collections in many areas of this region have prevented an accurate assessment of their genetic profile and structure for genetic improvement purposes.
In the present work, the genetic profile was estimated of wild accessions of C. papaya collected in Yucatan State using amplified fragment length polymorphism (AFLP) markers.
Eighteen wild accessions and one commercial accession of papaya were analyzed with 12 combinations of EcoRI-MseI primers.
Genetic variability among the wild accessions of C. papaya was estimated through pairwise comparison of genetic similarity.
It was observed that the differences between accessions at the DNA level were large, with an average genetic similarity of 0.13, and showed a range of 0.005 to 0.59. A total of 1432 polymorphic fragments were generated and scored as binary data.
The evaluation of genetic profiles among wild accessions of C. papaya provides useful information to estimate the potential for the use of marker-assisted for genetic improvement of C. papaya cultivars.
In the present work, the genetic profile was estimated of wild accessions of C. papaya collected in Yucatan State using amplified fragment length polymorphism (AFLP) markers.
Eighteen wild accessions and one commercial accession of papaya were analyzed with 12 combinations of EcoRI-MseI primers.
Genetic variability among the wild accessions of C. papaya was estimated through pairwise comparison of genetic similarity.
It was observed that the differences between accessions at the DNA level were large, with an average genetic similarity of 0.13, and showed a range of 0.005 to 0.59. A total of 1432 polymorphic fragments were generated and scored as binary data.
The evaluation of genetic profiles among wild accessions of C. papaya provides useful information to estimate the potential for the use of marker-assisted for genetic improvement of C. papaya cultivars.
Publication
Authors
A.C. Chan-León, A. Quiroz Moreno, G. Fuentes Ortíz, F.L. Espadas-Gil, C.R. Talavera-May, G. Montalvo Fernández, J. Ramírez-Prado, O. Zapata-Pérez, J.M. Santamaría
Keywords
AFLP markers, genetic similarity, polymorphism, sequencing by capillary
Groups involved
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