Articles
Genetic diversity of longan cultivars in Vietnam
Article number
1293_15
Pages
105 – 112
Language
English
Abstract
Currently, the identification of longan cultivars is mainly based on their morphological characters, homonymies and synonymies which is one of the limitations in longan breeding, germplasm collection, management and conservation.
To accelerate longan cultivar identification, the genetic diversity of 29 longan accessions that were conserved in the Southern Horticultural Research Institute (SOFRI) was characterized by microsatellite markers (simple sequence repeats – SSRs markers). Twenty-nine SSR makers that used to be applied for studying litchi diversity were again exploited to characterize molecular polymorphisms of the 29 longan accessions (26 collected from 7 provinces in Vietnam, one from Malaysia, one from China and the last from Thailand). A total of 84 amplification fragments were detected with an average of 2.9 alleles per SSR locus.
Over the eight analyzed single locus SSRs, the heterozygosity was expected at 0.46 while the observed heterozygosity was noted only 0.04 after polymorphism analysis.
Molecular characterization revealed the existence of 28 different genetic profiles.
Synonymy and homonymy in longan cultivars were found in ‘Bay To’ longan and ‘Soc Trang’ longan.
This study provided the fundamental information for the molecular characteristics and the genetic diversity of longan cultivars in Vietnam.
To accelerate longan cultivar identification, the genetic diversity of 29 longan accessions that were conserved in the Southern Horticultural Research Institute (SOFRI) was characterized by microsatellite markers (simple sequence repeats – SSRs markers). Twenty-nine SSR makers that used to be applied for studying litchi diversity were again exploited to characterize molecular polymorphisms of the 29 longan accessions (26 collected from 7 provinces in Vietnam, one from Malaysia, one from China and the last from Thailand). A total of 84 amplification fragments were detected with an average of 2.9 alleles per SSR locus.
Over the eight analyzed single locus SSRs, the heterozygosity was expected at 0.46 while the observed heterozygosity was noted only 0.04 after polymorphism analysis.
Molecular characterization revealed the existence of 28 different genetic profiles.
Synonymy and homonymy in longan cultivars were found in ‘Bay To’ longan and ‘Soc Trang’ longan.
This study provided the fundamental information for the molecular characteristics and the genetic diversity of longan cultivars in Vietnam.
Authors
T.T.O. Yen, D.T.T. Thao, T.T.T. Nhu, N.N. Truong, D.T.B. Bay, N.V. Hoa
Keywords
genetic diversity, longan accessions, microsatellites, polymorphism
Online Articles (37)