Articles
In vitro micrografting of selected Galician chestnut cultivars on chestnut hybrids (Castanea × coudercii)
Article number
1400_29
Pages
245 – 252
Language
English
Abstract
The high demand for grafted chestnut (Castanea sativa) trees in Galicia makes it necessary to develop new production strategies and to deepen the knowledge of compatibility of selected cultivars with Eurasian hybrids resistant to ink disease, used as rootstocks.
First results of in vitro micrografting of four chestnut cultivars on four artificial hybrid rootstocks were obtained in our laboratory, which permitted to assess short-term affinity on unrooted rootstocks, and evaluate in vitro micrografting to produce grafted plants.
For the production of micrografted plants, two strategies have been compared: 1) use of unrooted rootstocks subjected to rooting induction after micrografting, and 2) use of rooted rootstocks.
In the latter, we propose a simple method to sidestep the extraction of the root system from the medium during the micrografting procedure, which facilitates the procedure and reduces contamination risks.
The initial results show that grafting success in most scion/rootstock combinations is high (usually over 70%). Regarding production of micrografted plants, micrografting on unrooted rootstocks followed by rooting induction was partially successful in one of the cultivars (Negral), when axillary explants were used as scions.
The second strategy (using rooted rootstocks) proved to be useful, especially if the objective is the acclimation and production of grafted plants.
First results of in vitro micrografting of four chestnut cultivars on four artificial hybrid rootstocks were obtained in our laboratory, which permitted to assess short-term affinity on unrooted rootstocks, and evaluate in vitro micrografting to produce grafted plants.
For the production of micrografted plants, two strategies have been compared: 1) use of unrooted rootstocks subjected to rooting induction after micrografting, and 2) use of rooted rootstocks.
In the latter, we propose a simple method to sidestep the extraction of the root system from the medium during the micrografting procedure, which facilitates the procedure and reduces contamination risks.
The initial results show that grafting success in most scion/rootstock combinations is high (usually over 70%). Regarding production of micrografted plants, micrografting on unrooted rootstocks followed by rooting induction was partially successful in one of the cultivars (Negral), when axillary explants were used as scions.
The second strategy (using rooted rootstocks) proved to be useful, especially if the objective is the acclimation and production of grafted plants.
Publication
Authors
J.L. Fernández-Lorenzo, A.N. Prado, A. Couso, N. Ferreiro, R. Mosquera-Losada, A. Rigueiro
Keywords
Castanea sativa, compatibility, unrooted rootstocks, rooting, double-tube
Groups involved
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