Articles
MICROPROPAGATION OF SELECTED LEUCOSPERMUM CORDIFOLIUM: EFFECT OF ANTIBIOTICS AND GA3
Article number
316_9
Pages
55 – 58
Language
Abstract
The establishment of a dwarf clone of Leucospermum cordifolium in culture was difficult because of heavy endogenous bacterial contamination.
Two-nodal segments were placed on half strength Murashige and Skoog medium supplemented with 6-benzyl-aminopurine to which the antibiotics streptomycin and rifampicin were added.
The addition of 100 mg.l-1 rifampicin to the growth medium was most effective in suppressing the bacteria and enabling the growth of the Leucospermum. GA3 at 1–2 mg.l-1 was found to be essential for rapid shoot proliferation and elongation.
Shoots multiplied at the rate of approximately five new shoots within 8 weeks.
The best rooting was achieved when 1 mg.l-1 indole butyric acid (IBA) was added to the medium.
Relatively high light intensities promoted in vitro rooting.
Following rooting, plants were successfully transferred to outdoor conditions.
Two-nodal segments were placed on half strength Murashige and Skoog medium supplemented with 6-benzyl-aminopurine to which the antibiotics streptomycin and rifampicin were added.
The addition of 100 mg.l-1 rifampicin to the growth medium was most effective in suppressing the bacteria and enabling the growth of the Leucospermum. GA3 at 1–2 mg.l-1 was found to be essential for rapid shoot proliferation and elongation.
Shoots multiplied at the rate of approximately five new shoots within 8 weeks.
The best rooting was achieved when 1 mg.l-1 indole butyric acid (IBA) was added to the medium.
Relatively high light intensities promoted in vitro rooting.
Following rooting, plants were successfully transferred to outdoor conditions.
Authors
E. Tal, H. Solomon, J. Ben-Jaacov, A.A. Watad
Keywords
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