Articles
THE USE OF A LIQUID CULTURE SYSTEM FOR TEACHING MICROPROPAGATION IN SCHOOLS AND COLLEGES AND SOME RELATED EXPERIMENTS.
Article number
350_42
Pages
267 – 278
Language
Abstract
A liquid culture system has been successfully used by college horticultural students to micropropagate Clematis. In the system, explants were supported by Sorbarod cellulose plugs contained in cartridges standing in liquid medium and placed in a purpose made channelled culture box.
Shoots taken from Clematis cultures were successfully rooted into plugs immersed in sucrose free medium.
In subsequent class experiments, the influence of abscisic acid (ABA) on stomata and transpiration rates of leaves taken from control and micropropagated plants was compared.
ABA reduced stomatal opening proportionately more in leaves taken from the liquid culture system than in leaves from agar based systems, but less than that observed in the control stockplants.
These differences were examined with respect to the possible role of calcium as a second messenger in the ABA response, and the likelihood that the liquid culture system allowed higher levels of calcium to accumulate in micropropagated leaves.
Shoots taken from Clematis cultures were successfully rooted into plugs immersed in sucrose free medium.
In subsequent class experiments, the influence of abscisic acid (ABA) on stomata and transpiration rates of leaves taken from control and micropropagated plants was compared.
ABA reduced stomatal opening proportionately more in leaves taken from the liquid culture system than in leaves from agar based systems, but less than that observed in the control stockplants.
These differences were examined with respect to the possible role of calcium as a second messenger in the ABA response, and the likelihood that the liquid culture system allowed higher levels of calcium to accumulate in micropropagated leaves.
Authors
P.J. Orton
Keywords
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