Articles
EFFECT OF DIFFERENT FACTORS ON IN VITRO MULTIPLICATION OF LEUCADENDRON ‘SAFARI SUNSET’ (PROTEACEAE)
Article number
387_13
Pages
115 – 120
Language
Abstract
Some factors affecting the multiplication phase of micropropagation of Leucadendron ‘Safari Sunset’ through nodal segments were studied.
The presence of 6-benzyladenine (BA) in a multiplication medium resulted in a higher proliferation rate than kinetin or 2-isopentenyl-adenine (2-iP). The use of a mixture containing BA and kinetin produced a synergistic effect on bud formation.
The presence of naphthaleneacetic acid (NAA), indole-butyric acid (IBA) or gibberellic acid in the proliferation medium containing BA, did not significantly affect the formation of new buds per explant and its growth.
The MS medium at half strength produced better bud formation than ¾ MS and complete MS media.
The presence of activated charcoal in the medium clearly inhibited bud proliferation while the use of phytagel as agar substitute gelling agent produced a significant increase of bud multiplication and growth.
The presence of 6-benzyladenine (BA) in a multiplication medium resulted in a higher proliferation rate than kinetin or 2-isopentenyl-adenine (2-iP). The use of a mixture containing BA and kinetin produced a synergistic effect on bud formation.
The presence of naphthaleneacetic acid (NAA), indole-butyric acid (IBA) or gibberellic acid in the proliferation medium containing BA, did not significantly affect the formation of new buds per explant and its growth.
The MS medium at half strength produced better bud formation than ¾ MS and complete MS media.
The presence of activated charcoal in the medium clearly inhibited bud proliferation while the use of phytagel as agar substitute gelling agent produced a significant increase of bud multiplication and growth.
Publication
Authors
J.F. Pérez-Francés, A.J. Expósito, J.A. Rodríguez
Keywords
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