Articles
BIOTECHNOLOGICAL APPROACHES FOR THE IMPROVEMENT OF CAVENDISH BANANAS
Article number
490_42
Pages
415 – 424
Language
Abstract
Dessert bananas of the Cavendish group (AAA) are among the most important bananas grown in the subtropics.
For their improvement, genetic engineering is proposed which relies on fast growing in vitro cultures.
Therefore, firstly the storage potential of Cavendish clones under slow growth conditions is discussed.
Secondly, this stock material is tested for the presence of endogenous bacteria.
Thirdly, bacteria-free cultures are then turned into highly proliferating meristem cultures to obtain high quality scalps for the production of embryogenic cell suspensions.
After bombardment with the particle-inflow gun, transgenic cultures are selected and regenerated.
Preliminary results are presented on the expression of foreign genes in the bunch of a flowering ‘Williams’ banana, as well as in the leaves of the mother plant, daughter and granddaughter suckers.
For their improvement, genetic engineering is proposed which relies on fast growing in vitro cultures.
Therefore, firstly the storage potential of Cavendish clones under slow growth conditions is discussed.
Secondly, this stock material is tested for the presence of endogenous bacteria.
Thirdly, bacteria-free cultures are then turned into highly proliferating meristem cultures to obtain high quality scalps for the production of embryogenic cell suspensions.
After bombardment with the particle-inflow gun, transgenic cultures are selected and regenerated.
Preliminary results are presented on the expression of foreign genes in the bunch of a flowering ‘Williams’ banana, as well as in the leaves of the mother plant, daughter and granddaughter suckers.
Publication
Authors
R. Swennen, I. Van den houwe, S. Remy, L. Sági, H. Schoofs
Keywords
Cavendish, embryogenic cell suspension, gene expression, genetic transformation, meristem
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