PHYLOGENETIC ANALYSES OF THE SUBGENUS EUROSA USING THE ITS NRDNA SEQUENCE

We investigated the molecular systematics of the subgenus Eurosa based on the internal transcribed spacer (ITS) region of 18S-26S nuclear ribosomal DNA (nrDNA). We sequenced ITS and 5.8S coding regions of 16 taxa in the genus Rosa. Even though the Rosa species used were not recent in origin and were not derived from recent hybridization, several Rosa taxa contained many polymorphic nucleotide sites in the ITS regions of their nrDNA. The results of our study are mostly congruent with the conclusions drawn from RFLP and matK sequence analyses of chloroplast DNA (Matsumoto, et al., 1997, Matsumoto, et al., 1998). R. spinosissima var. pimpinellifoliae in section Pimpinellifoliae and R. carolina in section Carolinae formed a clade with 71% bootstrap replication.

XXV International Horticultural Congress, Part 11: Application of Biotechnology and Molecular Biology and Breeding – Gene Expression and Molecular Breeding, Genome Analysis.

S. Matsumoto, M. Kouchi, H. Fukui, Y. Ueda

Rose, nrDNA, ITS, Phylogeny

https://doi.org/10.17660/ActaHortic.2000.521.21