Articles
INTERGENERIC TRANSFER OF NEMATODE RESISTANCE FROM RAPHANUS SATIVUS INTO THE BRASSICA NAPUS GENOME
Article number
539_16
Pages
129 – 134
Language
Abstract
The beet cyst nematode (Heterodera schachtii Schm.) causes major damage to plant crops in temperate climates and is difficult to control effectively because of the long persistence of its spores in the soil.
Oilseed rape (Brassica napus), while not seriously affected by this pest, possesses very little natural resistance and thus acts as an efficient host for the perpetuation of nematode populations; consequently it is generally unsuitable for crop rotations with highly susceptible crops like sugar beet.
To overcome this, intergeneric crosses were made between spring oilseed rape and nematode-resistant oil radish (Raphanus sativus) genotypes, using embryo rescue to overcome incompatibility barriers.
In three backcross generations, highly resistant progeny with a minimal Raphanus genome component were identified by genomic in situ hybridisation (GISH). Among the early backcross offspring was a BC3 plant with a monosomic, acrocentric addition chromosome.
This individual has been backcrossed once again and then selfed to produce a stable disomic addition line.
This will then be further backcrossed, with the ultimate aim of generating rapeseed lines containing the resistance on a small introgression with minimal genetic drag.
Oilseed rape (Brassica napus), while not seriously affected by this pest, possesses very little natural resistance and thus acts as an efficient host for the perpetuation of nematode populations; consequently it is generally unsuitable for crop rotations with highly susceptible crops like sugar beet.
To overcome this, intergeneric crosses were made between spring oilseed rape and nematode-resistant oil radish (Raphanus sativus) genotypes, using embryo rescue to overcome incompatibility barriers.
In three backcross generations, highly resistant progeny with a minimal Raphanus genome component were identified by genomic in situ hybridisation (GISH). Among the early backcross offspring was a BC3 plant with a monosomic, acrocentric addition chromosome.
This individual has been backcrossed once again and then selfed to produce a stable disomic addition line.
This will then be further backcrossed, with the ultimate aim of generating rapeseed lines containing the resistance on a small introgression with minimal genetic drag.
Authors
A. Voss, R.J. Snowdon, W. Lühs
Keywords
Brassica napus, Raphanus sativus, Heterodera schachtii, rapeseed, nematode resistance, GISH, interspecific hybrids
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