Articles
IDENTIFICATION OF LOQUAT CULTIVARS BY FRUIT, LEAF AND ENDOSPERM ISOZYMES
Article number
598_25
Pages
173 – 180
Language
English
Abstract
In this experiment, genetic differences of 18 cultivars and/or ecotypes were detected by isozymes.
Mature leaves, fruits and endosperms of loquat cultivars and some of their ecotypes were obtained from Citrus and Greenhouse Crops Research Institute in Antalya, Turkey.
All samples were taken early in the morning and analysed in the same day.
By using vertical polyacrylamid slab gel electrophoresis (PAGE), isozymes banding patterns for alcohol dehydrogenase (ADH), catechol oxidase (CO), glutamate oxaloacetate transaminase (GOT), acide phosphatase (HP), and peroxidase (PER) in fruit and endosperms were analysed.
All enzyme systems were also analysed in the leaves but ADH was not detected in this plant tissue.
As a result of this experiment, for ADH, all cultivars had the same banding patterns in fruits.
Some biotypes within the same cultivars such as Yuvarlak Çukurgöbek and Tanaka had different banding patterns of endosperm.
In CO enzyme system extracted from leaves, fruits and endosperms, had different banding patterns.
All cultivars had the same banding of GOT in each of the plant tissue.
But band patterns changed among tissues.
In HP, three patterns were observed in the endosperm.
On the other hand, leaves and fruits of loquat cultivars had only one isozyme pattern with five bands.
There were one banding pattern with two bands for PER in loquat fruits, while three distinct banding patterns were observed in endosperms and leaves.
Akko 1 and Akko 13 can be easily distinguished with PER isozymes of leaves, whereas Tanaka and Yuvarlak Çukurgöbek had the same banding patterns for PER in the each of three plant tissues.
Isozymic differences between cultivars suggested that isozymes may provide useful markers for cultivars identification in loquat.
Mature leaves, fruits and endosperms of loquat cultivars and some of their ecotypes were obtained from Citrus and Greenhouse Crops Research Institute in Antalya, Turkey.
All samples were taken early in the morning and analysed in the same day.
By using vertical polyacrylamid slab gel electrophoresis (PAGE), isozymes banding patterns for alcohol dehydrogenase (ADH), catechol oxidase (CO), glutamate oxaloacetate transaminase (GOT), acide phosphatase (HP), and peroxidase (PER) in fruit and endosperms were analysed.
All enzyme systems were also analysed in the leaves but ADH was not detected in this plant tissue.
As a result of this experiment, for ADH, all cultivars had the same banding patterns in fruits.
Some biotypes within the same cultivars such as Yuvarlak Çukurgöbek and Tanaka had different banding patterns of endosperm.
In CO enzyme system extracted from leaves, fruits and endosperms, had different banding patterns.
All cultivars had the same banding of GOT in each of the plant tissue.
But band patterns changed among tissues.
In HP, three patterns were observed in the endosperm.
On the other hand, leaves and fruits of loquat cultivars had only one isozyme pattern with five bands.
There were one banding pattern with two bands for PER in loquat fruits, while three distinct banding patterns were observed in endosperms and leaves.
Akko 1 and Akko 13 can be easily distinguished with PER isozymes of leaves, whereas Tanaka and Yuvarlak Çukurgöbek had the same banding patterns for PER in the each of three plant tissues.
Isozymic differences between cultivars suggested that isozymes may provide useful markers for cultivars identification in loquat.
Authors
H.I. Uzun, I. Polat, S. Demir
Keywords
loquat, leaf, fruit, endosperm, isoenzyme, PAGE
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