Articles
EXPRESSION ANALYSIS OF GENES ENCODING AQUAPORINS DURING THE DEVELOPMENT OF PEACH FRUIT
Article number
618_42
Pages
363 – 370
Language
English
Abstract
To clarify the mechanisms of controlling water transport in peach fruit, the expression pattern of aquaporin genes (Pr-gTIP1, Pr-dTIP1, Pr-PIP1 and Pr-PIP2) were investigated.
According to genome gel DNA blot analysis, each of those genes exist as a single copy in the peach genome.
The amino acid sequence of Pr-gTIP1, deduced from the full sequence of the cDNA, was highly homologous to those of tonoplast intrinsic proteins (TIPs) isolated from other plants.
RNA gel blot analysis revealed that Pr-PIP1, belonging to a PIP family, is constitutively expressed throughout development of fruits, while other aquaporins such as Pr-gTIP belonging to γTIP was mainly expressed at an early stage of fruit growth.
The expression of Pr-gTIP1, Pr-dTIP1 and Pr-PIP2 decreased in response to drought stress, whereas that of Pr-PIP1 was not affected by the stress.
These results suggest membrane permeability may be controlled by the down regulation of these aquaporins.
According to genome gel DNA blot analysis, each of those genes exist as a single copy in the peach genome.
The amino acid sequence of Pr-gTIP1, deduced from the full sequence of the cDNA, was highly homologous to those of tonoplast intrinsic proteins (TIPs) isolated from other plants.
RNA gel blot analysis revealed that Pr-PIP1, belonging to a PIP family, is constitutively expressed throughout development of fruits, while other aquaporins such as Pr-gTIP belonging to γTIP was mainly expressed at an early stage of fruit growth.
The expression of Pr-gTIP1, Pr-dTIP1 and Pr-PIP2 decreased in response to drought stress, whereas that of Pr-PIP1 was not affected by the stress.
These results suggest membrane permeability may be controlled by the down regulation of these aquaporins.
Authors
S. Sugaya, I. Ohshima, H. Gemma, S. Iwahori
Keywords
aquaporin, drought stress, fruit, peach, Prunus persica
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