Home > XXVI International Horticultural Congress: The Future for Medicinal and Aromatic Plants > MASS PROPAGATION OF ECHINACEA ANGUSTIFOLIA: A PROTOCOL REFINEMENT USING SHOOT ENCAPSULATION AND TEMPORARY IMMERSION LIQUID SYSTEM

Articles

MASS PROPAGATION OF ECHINACEA ANGUSTIFOLIA: A PROTOCOL REFINEMENT USING SHOOT ENCAPSULATION AND TEMPORARY IMMERSION LIQUID SYSTEM

Article number

629_53

Pages

409 – 414

Language

English

Abstract

Tissue culture of Echinacea angustifolia allows for selection and rapid multiplication of genetically superior clones.
In this study, temporary immersion liquid bioreactor (RITA® system), synthetic seed technology and semi-solid culture conditions were evaluated for shoot multiplication efficiency.
After 25 days of culture, the highest multiplication rate was observed in explants cultured in temporary immersion liquid system.
Previous reports on micropropagation of E. angustifolia have had low success rate in rooting.
Shoots were rooted under autotrophic conditions on sugar-free semi-solid media supplemented with 2.5 µM IBA and using synthetic seed technology by shoot encapsulation in calcium alginate gel cultured on MS medium supplemented with 2.6 µM NAA. Rooting response was noticed in three weeks with a success rate of 83%. Arbuscular mycorrhiza infection helped the acclimatization survival rate of rooted plantlets.

Publication

XXVI International Horticultural Congress: The Future for Medicinal and Aromatic Plants

Authors

H. Lata, E. Bedir, R.M. Moraes, Z. Andrade

Keywords

Echinacea, tissue culture, micropropagation, RITA® bioreactor

Full text

https://doi.org/10.17660/ActaHortic.2004.629.53

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