Articles
EFFECT OF GROWTH REGULATORS ON HUPERZIA LUCIDULUM EXPLANTS IN VITRO
Article number
631_23
Pages
181 – 185
Language
English
Abstract
In vitro, Huperzia lucidulum shoot-tips grow slowly and do not readily form roots, although gemmae produce roots within 4-6 weeks (Waegel, in press). Experiments were designed to see if shoot growth and root formation could be accelerated with the growth regulators NAA (napthaleneacetic acid), BA-6 (benzyladenine) or both.
Club moss shoot-tips grew more in the presence of growth regulators compared to the control group, but statistical analysis of the growth data shows that the difference was not significant (p=0.56) There was a significant difference in the growth of shoot-tips and gemmae (p=.0001), even though gemmae had roots.
Rooting of shoot-tips was not stimulated by NAA and/or 6-BA. Of 20 H. lucidulum shoot tip cuttings maintained in culture for one year, only 3 formed roots.
Further experiments are needed to understand the factors involved in the rooting of club moss explants in vitro.
Club moss shoot-tips grew more in the presence of growth regulators compared to the control group, but statistical analysis of the growth data shows that the difference was not significant (p=0.56) There was a significant difference in the growth of shoot-tips and gemmae (p=.0001), even though gemmae had roots.
Rooting of shoot-tips was not stimulated by NAA and/or 6-BA. Of 20 H. lucidulum shoot tip cuttings maintained in culture for one year, only 3 formed roots.
Further experiments are needed to understand the factors involved in the rooting of club moss explants in vitro.
Authors
T. Dwyer, A.S. Waegel
Keywords
shining club moss, napthaleneacetic acid, benzyladenine, axenic culture, shoot-tips, gemmae
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