Articles
IN VITRO STORAGE OF HOP (HUMULUS L.) GERMPLASM
Article number
668_32
Pages
249 – 256
Language
English
Abstract
In this study we characterized the response of 70 diverse Humulus L. genotypes from the USDA-ARS National Clonal Germplasm Repository, Corvallis, Oregon, to in vitro storage under low light at 4° C and 28 to cryopreservation in liquid nitrogen by slow cooling.
Plantlets were cold acclimated, stored in plastic tissue-culture bags, and evaluated at 4 mo intervals.
The average storage time without transfer for the 70 genotypes evaluated was 14.1 ± 3.5 mo with a range of 6 to 26 mo.
Eight accessions stored for 2 cycles averaged 14.3 ± 3.2 months storage.
Plantlets for cryopreservation were cold acclimated for 2 wks, pretreated for 48 hrs, cooled to -40° C at 1° C per min, and plunged into liquid nitrogen.
Mean recovery of cryopreserved shoot tips of accessions was 54% ± 13 with 2-wk cold acclimation.
A few genotypes required longer cold acclimation for good recovery following liquid nitrogen exposure.
Plantlets were cold acclimated, stored in plastic tissue-culture bags, and evaluated at 4 mo intervals.
The average storage time without transfer for the 70 genotypes evaluated was 14.1 ± 3.5 mo with a range of 6 to 26 mo.
Eight accessions stored for 2 cycles averaged 14.3 ± 3.2 months storage.
Plantlets for cryopreservation were cold acclimated for 2 wks, pretreated for 48 hrs, cooled to -40° C at 1° C per min, and plunged into liquid nitrogen.
Mean recovery of cryopreserved shoot tips of accessions was 54% ± 13 with 2-wk cold acclimation.
A few genotypes required longer cold acclimation for good recovery following liquid nitrogen exposure.
Publication
Authors
B.M. Reed
Keywords
cryopreservation, cold acclimation, cold storage, Humulus lupulus, liquid nitrogen, tissue culture
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