Articles
USE OF A CHILEAN NATIVE STRAIN ‘SHERWOOD’ OF TRICHODERMA VIRENS ON THE BIOCONTROL OF BOTRYTIS CINEREA IN LETTUCES GROWN BY A FLOAT SYSTEM
Article number
697_56
Pages
437 – 440
Language
English
Abstract
The biocontrol activity of the native strain Sherwood of Trichoderma virens on Botrytis cinerea was estimated under a lettuce-growing hydroponic float system and compared with the commercial formulation of T. harzianum strain T39 (Trichodex), and the usual fungicide mixture, benomyl + captan.
Therefore, butterhead lettuce seedlings, cv.
Esmeralda, were grown on a floating system with the Wye nutrient solution under an environmental controlled greenhouse at Panguilemo Experimental Station, Universidad de Talca, Chile.
In a completely randomized block design, six treatments with three replications were used, so for each experimental unit, 36 plants were artificially inoculated with 106 conidia ml-1 of B. cinerea. Previously, the plants were sprayed with 109 conidia ml-1 of Sherwood strain, Trichodex or benomyl + captan, according to the planned treatments.
The incidence of grey rot in lettuce plants was evaluated at harvest time, 40 days after transplanting.
In addition, quality parameters like plant height, weight and number of commercial plants were also evaluated.
Lettuces treated with Trichoderma virens Sherwood showed a significant lower incidence (P<0.01) of gray rot disease and a significant higher percent of commercial plants (P<0.05) than the control ones.
The Sherwood strain, Trichodex and the fungicide mixture benomyl + captan showed a control activity on B. cinerea, but no significant differences were found among those treatments.
Nevertheless, when lettuce was treated with the special mixture between fungicides and the Sherwood strain, a significant higher percent (P<0.05) of commercial plants was observed.
Therefore, butterhead lettuce seedlings, cv.
Esmeralda, were grown on a floating system with the Wye nutrient solution under an environmental controlled greenhouse at Panguilemo Experimental Station, Universidad de Talca, Chile.
In a completely randomized block design, six treatments with three replications were used, so for each experimental unit, 36 plants were artificially inoculated with 106 conidia ml-1 of B. cinerea. Previously, the plants were sprayed with 109 conidia ml-1 of Sherwood strain, Trichodex or benomyl + captan, according to the planned treatments.
The incidence of grey rot in lettuce plants was evaluated at harvest time, 40 days after transplanting.
In addition, quality parameters like plant height, weight and number of commercial plants were also evaluated.
Lettuces treated with Trichoderma virens Sherwood showed a significant lower incidence (P<0.01) of gray rot disease and a significant higher percent of commercial plants (P<0.05) than the control ones.
The Sherwood strain, Trichodex and the fungicide mixture benomyl + captan showed a control activity on B. cinerea, but no significant differences were found among those treatments.
Nevertheless, when lettuce was treated with the special mixture between fungicides and the Sherwood strain, a significant higher percent (P<0.05) of commercial plants was observed.
Authors
M. Lolas, E. Donoso, V. Gonzáles, G. Carrasco
Keywords
biological control, soilless culture, leafy vegetables, sustainable production
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