Articles
A PRELIMINARY ANALYSIS OF DIVERSITY AMONG EAST AFRICAN SWEETPOTATO LANDRACES USING MORPHOLOGICAL AND SIMPLE SEQUENCE REPEATS (SSR) MARKERS
Article number
703_18
Pages
159 – 164
Language
English
Abstract
East Africa is known to have a wide range of sweetpotato landraces and, therefore, is considered to be a secondary center of diversity.
In this study, we assessed the diversity among sweetpotato landraces from Kenya, Uganda and Tanzania using morphological and simple sequence repeats (SSR) markers.
Two hundred and sixty-six landraces collected from agro-ecologically-distinct locations were screened for morphological characters using the CIP Research Guide. Morphological characters were recorded and phylogenetic analysis using UPGMA showed a close relatedness amongst the East African sweetpotato landraces with the majority having a 0.1-0.5 range of Neis genetic distance from each other.
The landraces, however, formed two major sub-clusters, irrespective of geographical origin.
Based on the morphological analysis, 57 landraces that were fairly distant were further analysed using four SSR primers specific for sweetpotato.
Each primer pair was able to generate between two and five polymorphic and clearly scorable fragments.
Phylogenetic analysis using UPGMA revealed similar results for the morphological characters.
However, in each analysis, landraces from Tanzania tended to cluster together, suggesting that they are morphologically and genetically distinct from the Kenyan and Ugandan accessions.
AMOVA, performed to further examine the relationships, indicated greater diversity than revealed by phylogenetic analysis suggesting that SSR markers are more reliable in assessing genetic diversity.
In this study, we assessed the diversity among sweetpotato landraces from Kenya, Uganda and Tanzania using morphological and simple sequence repeats (SSR) markers.
Two hundred and sixty-six landraces collected from agro-ecologically-distinct locations were screened for morphological characters using the CIP Research Guide. Morphological characters were recorded and phylogenetic analysis using UPGMA showed a close relatedness amongst the East African sweetpotato landraces with the majority having a 0.1-0.5 range of Neis genetic distance from each other.
The landraces, however, formed two major sub-clusters, irrespective of geographical origin.
Based on the morphological analysis, 57 landraces that were fairly distant were further analysed using four SSR primers specific for sweetpotato.
Each primer pair was able to generate between two and five polymorphic and clearly scorable fragments.
Phylogenetic analysis using UPGMA revealed similar results for the morphological characters.
However, in each analysis, landraces from Tanzania tended to cluster together, suggesting that they are morphologically and genetically distinct from the Kenyan and Ugandan accessions.
AMOVA, performed to further examine the relationships, indicated greater diversity than revealed by phylogenetic analysis suggesting that SSR markers are more reliable in assessing genetic diversity.
Publication
Authors
V. Gichuru, V. Aritua, G.W. Lubega, R. Edema, E. Adipala, P.R. Rubaihayo
Keywords
UPGMA, primers, AMOVA, polymorphism, CIP Research Guide
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