MOLECULAR BIOLOGY APPROACH FOR IMPROVING CHRYSANTHEMUM RESISTANCE TO VIRUS B

The chrysanthemum have been vegetatively propagated for a long time so the viruses and viroids which infect this group, have also been perpetuated.
Chrysanthemum virus B (CVB) is a member of the carlavirus group and it is widespread throughout the world in cultivated varieties of chrysanthemum.
Three expression vectors were constructed for Agrobacterium-mediated transformation of chrysanthemum (Chrysanthemum morifolium Ramat cv. ‘White Snowdon’) with the aim of improving the resistance to CVB infection.
These constructs contained the double 35S promoter, the kanamycin-resistance selectable marker gene and the nucleotide sequence of the open reading frame 5 (ORF5) encoding the CVB coat protein in sense, antisense and double sense orientation.
We obtained 12 transgenic chrysanthemum lines with antisense cDNA sequence, 7 lines with sense cDNA sequence and 20 lines with double sense cDNA sequence.
The integration of the coat protein gene in the plants was detected by PCR.

XXII International Eucarpia Symposium, Section Ornamentals, Breeding for Beauty

T.S. Skachkova, T.Y. Mitiouchkina, S.A. Taran, S.V. Dolgov

Chrysanthemum morifolium Ramat., virus resistance, coat protein, genetic transformation, chrysanthemum virus B, antisense RNA

https://doi.org/10.17660/ActaHortic.2006.714.21