Articles
IDENTIFICATION OF TWO 1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE GENES (CP-ACO1 AND CP-ACO2) FROM CARICA PAPAYA AND CHARACTERISATION OF PUTATIVE CP-ACO1 PROMOTER USING AGROBACTERIUM INFILTRATION
Article number
740_18
Pages
163 – 168
Language
English
Abstract
Two papaya 1-aminocyclopropane-1-carboxylate oxidase genes (CP-ACO1 and CP-ACO2) were cloned, sequenced and characterized.
The genes shared 58% identity at the nucleotide level.
Further, the 5flanking region of CP-ACO1 was isolated using gene specific and ligation mediated amplification.
The promoter activity of this CP-ACO1 5 flanking region (1405 bp) was studied in two ways; promoter identification via a database assisted bioinformatics approach and transient expression using Agrobacterium infiltration.
Several seed specific and ethylene responsive elements were subcloned into pCAMBIA vectors containing GUS and subsequently used for Agrobacterium infiltration experiments.
The results confirm seed and fruit specific promoter activity within 5 flanking region of CP-ACO1.
The genes shared 58% identity at the nucleotide level.
Further, the 5flanking region of CP-ACO1 was isolated using gene specific and ligation mediated amplification.
The promoter activity of this CP-ACO1 5 flanking region (1405 bp) was studied in two ways; promoter identification via a database assisted bioinformatics approach and transient expression using Agrobacterium infiltration.
Several seed specific and ethylene responsive elements were subcloned into pCAMBIA vectors containing GUS and subsequently used for Agrobacterium infiltration experiments.
The results confirm seed and fruit specific promoter activity within 5 flanking region of CP-ACO1.
Publication
Authors
P. Burns, S. Chubunmee, P. Pinon, O. Kumde
Keywords
ethylene, promoter analysis, ethylene responsive element, transient expression
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