Articles
GENETIC ANALYSIS OF PHYLLOXERA ROOT RESISTANCE IN CULTIVAR ‘BÖRNER’
Article number
904_6
Pages
47 – 52
Language
English
Abstract
Most rootstock cultivars currently used show root nodosity symptoms after phylloxera infection.
However, rootstock cultivar Börner, an interspecific cross of Vitis riparia 183G and Vitis cinerea Arnold, is regarded as phylloxera-resistant as it shows neither tuberosity nor nodosity symptoms.
A classical map-based cloning approach was used to identify the genetic determinants of phylloxera root resistance in Börner. The roots of the progeny of a cross of Gf.V.3125 (Schiava grossa × Riesling) × Börner were artificially infested with phylloxera leaf galls (containing phylloxera) and phenotyped qualitatively and quantitatively for nodosity formation.
After establishing a genetic map of the parents a single significant locus named Rdv1 (Resistance Daktulosphaira vitifoliae) was localized on chromosome 13 in the map of Börner (Zhang et al., 2009). Simple sequence repeat (SSR) marker comparison between Börner and the parents V. riparia 183G and V. cinerea Arnold confirmed the latter as the origin of Rdv1. Fine mapping of the Rdv1 locus was started with synteny-derived STS (sequence-tagged site) markers deduced from the PN40024 reference sequence.
Markers, tightly linked to the resistance locus, were obtained and found to be useful for marker-assisted selection in breeding programs.
However, rootstock cultivar Börner, an interspecific cross of Vitis riparia 183G and Vitis cinerea Arnold, is regarded as phylloxera-resistant as it shows neither tuberosity nor nodosity symptoms.
A classical map-based cloning approach was used to identify the genetic determinants of phylloxera root resistance in Börner. The roots of the progeny of a cross of Gf.V.3125 (Schiava grossa × Riesling) × Börner were artificially infested with phylloxera leaf galls (containing phylloxera) and phenotyped qualitatively and quantitatively for nodosity formation.
After establishing a genetic map of the parents a single significant locus named Rdv1 (Resistance Daktulosphaira vitifoliae) was localized on chromosome 13 in the map of Börner (Zhang et al., 2009). Simple sequence repeat (SSR) marker comparison between Börner and the parents V. riparia 183G and V. cinerea Arnold confirmed the latter as the origin of Rdv1. Fine mapping of the Rdv1 locus was started with synteny-derived STS (sequence-tagged site) markers deduced from the PN40024 reference sequence.
Markers, tightly linked to the resistance locus, were obtained and found to be useful for marker-assisted selection in breeding programs.
Publication
Authors
L. Hausmann, R. Eibach, E. Zyprian, R. Töpfer
Keywords
phylloxera, resistance, Vitis cinerea, mapping, marker
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