Articles
SIMPLE SPECTROGRAPHIC TECHNIQUE USING A FLUORESCENT DYE TO EVALUATE WATER-UPTAKE BY CUT FLOWER PEDUNCLES
Article number
919_4
Pages
35 – 38
Language
English
Abstract
A low-cost, simple spectrographic technique was developed using a commercial camera and fluorescent dye (excitation peak of wavelength: 494 nm, emission peak of wavelength: 521 nm) to monitor water uptake by a cut flower stem.
To obtain a clear image of fluorescence emission, the following combinations of light sources, exciter filters, and emitter filters were compared: (1) halogen light with a fiber illuminator for microscopes was tested against speedlight for a commercial camera, (2) a blue dichroic filter versus an exciter filter for fluorescent reagent GFP, and (3) band-pass filters with a center wavelength of 515 nm versus those with a center wavelength of 520 nm.
Cut rose flowers were used as samples.
The clearest image was obtained when a combina¬tion of speedlight, exciter filter for a fluorescence microscope, and emitter filter with a center wavelength at 520 nm was used.
To obtain a clear image of fluorescence emission, the following combinations of light sources, exciter filters, and emitter filters were compared: (1) halogen light with a fiber illuminator for microscopes was tested against speedlight for a commercial camera, (2) a blue dichroic filter versus an exciter filter for fluorescent reagent GFP, and (3) band-pass filters with a center wavelength of 515 nm versus those with a center wavelength of 520 nm.
Cut rose flowers were used as samples.
The clearest image was obtained when a combina¬tion of speedlight, exciter filter for a fluorescence microscope, and emitter filter with a center wavelength at 520 nm was used.
Authors
U. Matsushima, Y. Yamada, H. Shono , M. Okada
Keywords
storage, optical filter, freshness
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