Articles
PLANT REGENERATION AND SYNTHETIC SEEDS PRODUCTION OF BRASSICA OLERACEA VAR. ITALIC
Article number
958_21
Pages
179 – 185
Language
English
Abstract
Agricultural industry has changed tremendously, especially since the end of World War II. Food industry has blossomed due to new technology, addition of preservative usage and government policies that favoured maximizing productions.
Thus, sustainable vegetable production has captured the attention of many countries worldwide while at the same time preserving environmental quality and making efficient use of non-renewable resources. Brassica oleracea var. italic or commonly known as broccoli from the family Brassicaceae is an important vegetable.
Broccoli is a member of the cabbage family providing good source of vitamins A and C among other nutritional benefits.
Broccoli explants (peduncle, stem and root) were cultured on Murashige and Skoog (MS) medium with different concentrations and various combinations of growth hormones including α-naphthalene acetic acid (NAA), 6-benzylaminopurine (BAP), 2,4-dichlorophenoxy acetic acid (2,4-D) and Thidiazuron (TDZ) ranging from 0.1 to 3.0 mg/L. Multiple shoots were best observed in MS media with combinations of 3.0 mg/L BAP and 1.0 mg/L NAA. Further studies including synthetic seeds production were done.
Synthetic seeds were created by encapsulating multiple shoots with calcium alginate.
Multiple shoots which were encapsulated with calcium alginate supplemented with 3.0 mg/L BAP and 1.0 mg/L NAA gave the best result for germination (70%). These results showed that synthetic seeds have potential to manipulate in vitro culture systems for large scale production.
The ability to produce synthetic seeds of broccoli is essential in increasing Malaysias agricultural industry and economy.
Thus, sustainable vegetable production has captured the attention of many countries worldwide while at the same time preserving environmental quality and making efficient use of non-renewable resources. Brassica oleracea var. italic or commonly known as broccoli from the family Brassicaceae is an important vegetable.
Broccoli is a member of the cabbage family providing good source of vitamins A and C among other nutritional benefits.
Broccoli explants (peduncle, stem and root) were cultured on Murashige and Skoog (MS) medium with different concentrations and various combinations of growth hormones including α-naphthalene acetic acid (NAA), 6-benzylaminopurine (BAP), 2,4-dichlorophenoxy acetic acid (2,4-D) and Thidiazuron (TDZ) ranging from 0.1 to 3.0 mg/L. Multiple shoots were best observed in MS media with combinations of 3.0 mg/L BAP and 1.0 mg/L NAA. Further studies including synthetic seeds production were done.
Synthetic seeds were created by encapsulating multiple shoots with calcium alginate.
Multiple shoots which were encapsulated with calcium alginate supplemented with 3.0 mg/L BAP and 1.0 mg/L NAA gave the best result for germination (70%). These results showed that synthetic seeds have potential to manipulate in vitro culture systems for large scale production.
The ability to produce synthetic seeds of broccoli is essential in increasing Malaysias agricultural industry and economy.
Authors
A.I.M. Yussof, S.N.A. Wafa , R.M. Taha
Keywords
Brassica oleracea var. italic, broccoli, plant regeneration, callus, synthetic seeds
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