Articles
OCCURRENCE OF ALFALFA MOSAIC VIRUS (AMV) DISEASES ON POTATO CROPS IN NORTHERN CYPRUS
Article number
960_49
Pages
341 – 346
Language
English
Abstract
Alfalfa mosaic virus (AMV) was for the first time detected in potato, peppers and alfalfa crops growing in North Cyprus.
This virus was frequently found on the hosts nearby alfalfa plantation areas.
The detection and characterization of AMV was made by bioassay, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction (RT-PCR). The collected potato (154), alfalfa (81) and pepper (63) samples were proceeded to test by DAS ELISA and RT-PCR. RT-PCR tests using primers, specific for the coat protein gene of AMV, resulted in an amplicon of the expected size (351 bp). The amplicons were then direct sequenced and deposited in GenBank (JN104033, JN104034). Direct sequencing analyses showed that potato, alfalfa and pepper AMV virus isolates were highly similar to each other.
Sequence analysis (BioEdit v 7.0.5) and a comparison with other AMV isolates showed about 96% (nt) identities with FN667767 and HQ185596. Comparison of the isolates of AMV to each other and type of symptoms expression of viruses on three host plants revealed that potato, pepper and alfalfa plants were infected by the same AMV strains.
The results of serological and molecular assays clearly suggested that alfalfa plants is an over wintering host and source of inoculums of AMV. The virus from the infection source (the alfalfa fields) was transmitted by the migration of aphids at the nearby potato and pepper fields.
Latter on, AMV has been completed the life cycle on potato and pepper plants at the end of growing seasons.
This virus was frequently found on the hosts nearby alfalfa plantation areas.
The detection and characterization of AMV was made by bioassay, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction (RT-PCR). The collected potato (154), alfalfa (81) and pepper (63) samples were proceeded to test by DAS ELISA and RT-PCR. RT-PCR tests using primers, specific for the coat protein gene of AMV, resulted in an amplicon of the expected size (351 bp). The amplicons were then direct sequenced and deposited in GenBank (JN104033, JN104034). Direct sequencing analyses showed that potato, alfalfa and pepper AMV virus isolates were highly similar to each other.
Sequence analysis (BioEdit v 7.0.5) and a comparison with other AMV isolates showed about 96% (nt) identities with FN667767 and HQ185596. Comparison of the isolates of AMV to each other and type of symptoms expression of viruses on three host plants revealed that potato, pepper and alfalfa plants were infected by the same AMV strains.
The results of serological and molecular assays clearly suggested that alfalfa plants is an over wintering host and source of inoculums of AMV. The virus from the infection source (the alfalfa fields) was transmitted by the migration of aphids at the nearby potato and pepper fields.
Latter on, AMV has been completed the life cycle on potato and pepper plants at the end of growing seasons.
Publication
Authors
H. Fidan, N.A. Adak, A. Konuksal, E. Akerzurumlu, M.A. Yılmaz
Keywords
AMV, Potato, Pepper, Alfalfa, RT-PCR sequencing
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