Articles
MICROPROPAGATION IN VITRO OF OPUNTIA FICUS-INDICA IN SOUTHERN MOROCCO
Article number
995_11
Pages
93 – 98
Language
English
Abstract
With the aim of large production of plant material, a rapid micropropagation method of prickly pear cactus (Opuntia ficus-indica) was developed.
Young cladode explants containing one areole were cultured on Murashige and Skoog (1962) medium (MS) with adenine sulfate (40 mg/L), monosodium phosphate (50 mg/L), sucrose (50 g/L), phytagel (0.3%) and benzyladenine (BA) at 22.2 µM. Best multiplication rates were obtained with BA at 0.5 mg/L than kinetin.
To induce rooting, the proliferated shoots were transferred to a medium containing half MS, sucrose (3%), phytagel (0.3%) and 0.5 mg/L of different auxins.
In these culture conditions, 100% of plantlets rooted and highest number of root (21.05 and 16.2) was obtained respectively with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The rooted shoots were easily acclimated and transferred to soil.
Young cladode explants containing one areole were cultured on Murashige and Skoog (1962) medium (MS) with adenine sulfate (40 mg/L), monosodium phosphate (50 mg/L), sucrose (50 g/L), phytagel (0.3%) and benzyladenine (BA) at 22.2 µM. Best multiplication rates were obtained with BA at 0.5 mg/L than kinetin.
To induce rooting, the proliferated shoots were transferred to a medium containing half MS, sucrose (3%), phytagel (0.3%) and 0.5 mg/L of different auxins.
In these culture conditions, 100% of plantlets rooted and highest number of root (21.05 and 16.2) was obtained respectively with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The rooted shoots were easily acclimated and transferred to soil.
Authors
A. El Finti, R. El Boullani, M. Belayadi, N. Ait Aabd, A. El Mousadik
Keywords
prickly pear, Opuntia ficus-indica, in vitro micropropagation, auxins, micropropagated plantlets
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