Articles
ESTABLISHMENT OF A VIRUS ASSAY SYSTEM BY RT-PCR FOR NARCISSUS TAZETTA VAR. CHINENSIS AND THE DISTRIBUTION OF VIRUS INFECTION IN CULTURE FIELDS
Article number
1002_29
Pages
237 – 241
Language
English
Abstract
Among the viruses that infect Japanese narcissus (Narcissus tazetta var. chinensis) cultured in open fields, Narcissus latent virus (NLV), Narcissus mosaic virus (NMV) and Narcissus yellow stripe virus (NYSV) were selected to establish a virus detection system.
Primers with nearly identical Tm values specific to each virus were designed. ACTIN gene-specific primers were also designed for reference.
By mixing primers specific to NLV, NYSV, and the ACTIN gene, we easily detected these viruses using PCR. Leaf samples from three different areas of narcissus cultivation were analyzed.
NLV was detected in more than 90% of plants from all three areas regardless of whether visible viral symptoms were displayed.
The ratio of NYSV-infected plants was higher in the northern area.
Relative concentrations of NLV and NYSV based on ACTIN mRNA were different among culture fields, suggesting that cultivation methods influence virus propagation.
Primers with nearly identical Tm values specific to each virus were designed. ACTIN gene-specific primers were also designed for reference.
By mixing primers specific to NLV, NYSV, and the ACTIN gene, we easily detected these viruses using PCR. Leaf samples from three different areas of narcissus cultivation were analyzed.
NLV was detected in more than 90% of plants from all three areas regardless of whether visible viral symptoms were displayed.
The ratio of NYSV-infected plants was higher in the northern area.
Relative concentrations of NLV and NYSV based on ACTIN mRNA were different among culture fields, suggesting that cultivation methods influence virus propagation.
Authors
E. Hori , H. Sakamoto, S. Ohki, R. Imura , Y. Goshima
Keywords
Narcissus latent virus, Narcissus mosaic virus, Narcissus yellow stripe virus, actin mRNA
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