Articles
HIGH EFFICIENCY REGENERATION SYSTEM OF ORIENTAL LILY CULTIVAR FOR TRANSGENIC MANIPULATION
Article number
1002_32
Pages
257 – 261
Language
English
Abstract
An efficient in vitro protocol for micropropagation of Oriental lily cultivar Constanta has been established.
Adventitious buds were induced from bulblet-scale thin cell layers cultured on MS medium supplemented with cytokinin and auxin.
The effect of 6-BA and TDZ on adventitious bud induction from the bulblet-scale thin cell layers was investigated.
The concentration of 6-BA and TDZ strongly affected the bud forming capacity.
Also, the culture condition significantly influenced the adventitious bud formation.
Results showed that the optimal medium for adventitious bud induction is MS basal medium supplemented with 0.004 mg/L TDZ and 0.2 mg/L 2,4-D. The differentiation frequency and the average number of adventitious buds reached 98.89% and 6.53, respectively.
When the adventitious buds were sub-cultured two or three times, their circumference reached 3-4 cm.
The bulblets that were 3-4 cm in circumference were transplanted in the greenhouse for hardening and their survival percentage was 96% after 6 weeks.
This efficient and rapid regeneration system may also be helpful for Agrobacterium-mediated or particle gun-mediated genetic transformation for the important bulbous crops.
Adventitious buds were induced from bulblet-scale thin cell layers cultured on MS medium supplemented with cytokinin and auxin.
The effect of 6-BA and TDZ on adventitious bud induction from the bulblet-scale thin cell layers was investigated.
The concentration of 6-BA and TDZ strongly affected the bud forming capacity.
Also, the culture condition significantly influenced the adventitious bud formation.
Results showed that the optimal medium for adventitious bud induction is MS basal medium supplemented with 0.004 mg/L TDZ and 0.2 mg/L 2,4-D. The differentiation frequency and the average number of adventitious buds reached 98.89% and 6.53, respectively.
When the adventitious buds were sub-cultured two or three times, their circumference reached 3-4 cm.
The bulblets that were 3-4 cm in circumference were transplanted in the greenhouse for hardening and their survival percentage was 96% after 6 weeks.
This efficient and rapid regeneration system may also be helpful for Agrobacterium-mediated or particle gun-mediated genetic transformation for the important bulbous crops.
Authors
Mengli Xi, Shuai Qiu, Ye Lu , Jisen Shi
Keywords
micropropagation, Oriental lily cultivar ‘Constanta’, thin cell layer culture
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