Articles
EFFECTS OF TOTAL NITROGEN AND BA ON IN VITRO CULTURE OF PHALAENOPSIS
Article number
1025_35
Pages
243 – 245
Language
English
Abstract
The effects of total nitrogen and 6-benzyladenine (BA) on growth of Phalaenopsis orchid were studied. Phalaenopsis seedlings were cultured on Vacin and Went (VW) medium containing 4.5, 6, 7.5 or 9 mM total nitrogen in combination with 0, 5, 10, 15 or 20 mg L-1 BA for 5 months.
The results revealed that VW medium supplemented with 9 mM nitrogen and 10 mg L-1 BA gave the best leaf length, 3.4 cm, and width, 1.3 cm, with an average of three leaves per plantlet.
However, the greatest number of roots (six roots per plantlet) and root length
(3.9 cm), were found on the VW medium containing 6 mM nitrogen and 5 mg L-1 BA. All rooted plants were successfully acclimatized and potted in the greenhouse.
The results revealed that VW medium supplemented with 9 mM nitrogen and 10 mg L-1 BA gave the best leaf length, 3.4 cm, and width, 1.3 cm, with an average of three leaves per plantlet.
However, the greatest number of roots (six roots per plantlet) and root length
(3.9 cm), were found on the VW medium containing 6 mM nitrogen and 5 mg L-1 BA. All rooted plants were successfully acclimatized and potted in the greenhouse.
Authors
K. Kanchanapoom, T. Anuphan , S. Pansiri
Keywords
leaf length, leaf width, root length, moth orchid, VW medium
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