Articles
INDUCTION OF EMBRYOGENIC CALLUS AND FLORAL ORGAN ORGANOGENESIS DERIVED FROM CALLUS OF LILIUM ORIENTAL ‘SORBONNE’
Article number
1027_21
Pages
197 – 204
Language
English
Abstract
Floral explants of Lilium Oriental Sorbonne produce embryogenic callus on a Murashige and Skoog (MS) medium with different concentrations of 2,4-dichlorophenoxyacetic (2,4-D) and 6-benzyladenine (BA). Nurse culture is used when inducing callus from filaments and regeneration ability of callus is examined.
Floral organ organogenesis is discovered in all the explants including style, petal, pedicel and filament (with anther) although at a low rate.
Especially when it comes to filament, a relative complete flower forms after cultured for 120 days, of which floral organ organogenesis starts at the 70th day.
Adjustment of media formula and light conditions keeps the flower alive for 70 days before totally withering and producing regenerated plantlets.
These results could be a supplement to studies on flowering of geophytes in vitro.
In terms of callus induction and plant regeneration for genetic improvement of lily, style and pedicel are found to give the highest frequency of callus induction rate of 96%-100%. The style gave the biggest callus cluster size after cultured for 4 months, which reaches a diameter of 9.81 mm.
The pedicel showed the highest regeneration and proliferation rate of plantlets.
These results indicate that pedicel and style may be an alternative or even a better choice compared to filament for inducing callus from floral organs.
Anther and ovary development in vitro is also examined.
Scanning electron microscope examination indicates that pollen grain is malformed and seeds are wizened.
Floral organ organogenesis is discovered in all the explants including style, petal, pedicel and filament (with anther) although at a low rate.
Especially when it comes to filament, a relative complete flower forms after cultured for 120 days, of which floral organ organogenesis starts at the 70th day.
Adjustment of media formula and light conditions keeps the flower alive for 70 days before totally withering and producing regenerated plantlets.
These results could be a supplement to studies on flowering of geophytes in vitro.
In terms of callus induction and plant regeneration for genetic improvement of lily, style and pedicel are found to give the highest frequency of callus induction rate of 96%-100%. The style gave the biggest callus cluster size after cultured for 4 months, which reaches a diameter of 9.81 mm.
The pedicel showed the highest regeneration and proliferation rate of plantlets.
These results indicate that pedicel and style may be an alternative or even a better choice compared to filament for inducing callus from floral organs.
Anther and ovary development in vitro is also examined.
Scanning electron microscope examination indicates that pollen grain is malformed and seeds are wizened.
Publication
Authors
L. Zhang, Y.P. Xia
Keywords
Lilium Oriental ‘Sorbonne’, floral organ, callus, flowering in vitro, regeneration
Online Articles (25)