Articles
IDENTIFICATION AND CHARACTERIZATION OF A POTYVIRUS ASSOCIATED WITH SEVERE MOSAIC DISEASE OF OPIUM POPPY (PAPAVER SOMNIFERUM L.) IN INDIA
Article number
1036_15
Pages
141 – 149
Language
English
Abstract
Opium Poppy (Papaver somniferum L.) is an important medicinal plant, which is a rich source of several alkaloids having significant therapeutic values.
This crop is continuously being threatened by a severe mosaic disease.
The causative agent for this disease has been identified and characterized as a virus, which we named as Poppy mosaic virus (PMV-P) belonging to the family Potyviridae. The virus was found to have very restricted host range.
However, Argimone maxicana L. which is a common weed usually grown in the vicinity of the crop was found to be a susceptible systematic host.
The purified preparation showed flexuous rod shaped virus particles of about 750 nm in length.
High titer polyclonal antiserum was produced against this virus in rabbit.
Heterologous and homologous serological relationship was determined using DAC-ELISA, showing its relationship with another Potyvirus infecting chili pepper (PVBV). Cytopathological studies using electron microscope showed the presence of pinwheel inclusion bodies in the infected poppy cells.
Taken together, our studies strongly suggest the virus causing mosaic disease in opium poppy to be a member of the Potyviridae family.
This crop is continuously being threatened by a severe mosaic disease.
The causative agent for this disease has been identified and characterized as a virus, which we named as Poppy mosaic virus (PMV-P) belonging to the family Potyviridae. The virus was found to have very restricted host range.
However, Argimone maxicana L. which is a common weed usually grown in the vicinity of the crop was found to be a susceptible systematic host.
The purified preparation showed flexuous rod shaped virus particles of about 750 nm in length.
High titer polyclonal antiserum was produced against this virus in rabbit.
Heterologous and homologous serological relationship was determined using DAC-ELISA, showing its relationship with another Potyvirus infecting chili pepper (PVBV). Cytopathological studies using electron microscope showed the presence of pinwheel inclusion bodies in the infected poppy cells.
Taken together, our studies strongly suggest the virus causing mosaic disease in opium poppy to be a member of the Potyviridae family.
Publication
Authors
M. Zaim, J. Joseph, R.K. Lal
Keywords
Papaver somniferum L., Potyviridae, Potyvirus, PMV-P, HEPES buffer, DAC-ELISA, polymerase chain reaction
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