Articles
NOVEL INSIGHTS INTO THE REGULATION OF ETHYLENE BIOSYNTHESIS/ACTION: PROTEIN TURNOVER BY A RIPENING RELATED FBOX PROTEIN IN TOMATO
Article number
1048_21
Pages
179 – 186
Language
English
Abstract
FBOX proteins target either EIN3 (EBF1 and EBF2) or EIN2 (ETP1 and ETP2) for protein degradation in Arabidopsis thaliana. Here we report the functional characterization of a novel ripening related FBOX protein from tomato we have designated SlFBOX1. SlFBOX1 transcript levels peak during the later stages of ripening.
An RNAi SlFBOX1 construct, driven by the constitutive 35S CaMV promoter was deployed into tomato and the resulting transgenic plants were characterized. SlFBOX1 gene expression was lower in transgenic RNAi lines than in WT control plants.
Primary transgenic plants have delayed petal senescence, similar to the ethylene insensitive mutant NEVERRIPE (Nr). We hypothesize it regulates a novel mechanism for ethylene signaling or biosynthesis in tomato.
An RNAi SlFBOX1 construct, driven by the constitutive 35S CaMV promoter was deployed into tomato and the resulting transgenic plants were characterized. SlFBOX1 gene expression was lower in transgenic RNAi lines than in WT control plants.
Primary transgenic plants have delayed petal senescence, similar to the ethylene insensitive mutant NEVERRIPE (Nr). We hypothesize it regulates a novel mechanism for ethylene signaling or biosynthesis in tomato.
Authors
N. Gapper, R. Alba, J. Giovannoni
Keywords
tomato, fruit ripening, ethylene, protein turnover
Online Articles (28)