Articles
IN VITRO CHEMOTHERAPY OF PEAR CULTIVARS
Article number
1048_27
Pages
221 – 224
Language
English
Abstract
The aim of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Apple stem pitting virus (ASPV) from in vitro plants of infected pear cultivars Astra, David and Erika. According to RT-PCR testing, selected field-grown trees of all pear cultivars were infected with ASPV. In vitro cultures were successfully multiplied on MS medium with BAP (6-benzylaminopurine). Ribavirin was used at 20 mg L-1 for virus elimination.
Low phytotoxicity of ribavirin at 20 mg L-1 was observed only for the cultivar David. Six of the twenty shoot tips of David showed apex necrosis and died after transfer to regeneration medium.
The success of chemotherapy after RT-PCR testing of ribavirin treated plants varied between 65.0% for cultivars Astra and Erika and 71.4% for cultivar David. The results achieved and the relatively high percentage of virus free plants obtained after 6 months of in vitro cultivation confirm the effectiveness of ribavirin in eliminating ASPV in pear.
Low phytotoxicity of ribavirin at 20 mg L-1 was observed only for the cultivar David. Six of the twenty shoot tips of David showed apex necrosis and died after transfer to regeneration medium.
The success of chemotherapy after RT-PCR testing of ribavirin treated plants varied between 65.0% for cultivars Astra and Erika and 71.4% for cultivar David. The results achieved and the relatively high percentage of virus free plants obtained after 6 months of in vitro cultivation confirm the effectiveness of ribavirin in eliminating ASPV in pear.
Authors
F. Paprstein, J. Sedlak, L. Talacko
Keywords
ASPV, ELISA, PCR, virus detection, virus elimination, virus infection
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