Articles
EFFECT OF TEMPERATURE, SALT AND OSMOTIC STRESSES ON SEED GERMINATION AND CHLOROPHYLL CONTENTS IN LENTIL (LENS CULINARIS MEDIK)
Article number
1054_4
Pages
47 – 54
Language
English
Abstract
Prolonged exposure to high or low temperature, salinity and osmotic stress can result in retardation of growth, reduction in yield, and even death of plants.
Two Jordan cultivated lentil (Lens culinaris Medik) cultivars, Jordan 1 and 2, were tested for their tolerance to cold (4°C), heat (42°C), salt (25, 50, 100, and 150 mM NaCl) and osmotic stress (50, 100, 150, 200 and 400 mM mannitol and sorbitol) in terms of seeds germination, and seedlings chlorophyll content.
The Jordan 1 seeds showed significant sensitivity under all heat treatments at 42°C compared to the Jordan 2 seeds.
The germination percentage under various NaCl, mannitol and sorbitol concentrations were decreased and delayed in both lentil cultivars after
7 days of treatment.
Heat stress inhibited chlorophyll synthesis in both Jordan 1 and Jordan 2 cultivars.
The Jordan 2 cultivar showed lower inhibition in chlorophyll synthesis under cold and heat stress than the Jordan 1 cultivar.
The chl a and chl b contents of both cultivars decreased as the NaCl concentration increased.
Mannitol treatments caused higher reduction in the chlorophyll contents in Jordan 2 seedlings than Jordan 1 seedlings.
The total chlorophyll contents for both lentil cultivars were reduced under all sorbitol treatments.
The results of this study indicated a direct effect of cold, heat, salt and osmotic abiotic stresses on seed viability and photosynthetic pigments in both lentil (Lens culinaris Medik) cultivars that are grown in Jordan.
Two Jordan cultivated lentil (Lens culinaris Medik) cultivars, Jordan 1 and 2, were tested for their tolerance to cold (4°C), heat (42°C), salt (25, 50, 100, and 150 mM NaCl) and osmotic stress (50, 100, 150, 200 and 400 mM mannitol and sorbitol) in terms of seeds germination, and seedlings chlorophyll content.
The Jordan 1 seeds showed significant sensitivity under all heat treatments at 42°C compared to the Jordan 2 seeds.
The germination percentage under various NaCl, mannitol and sorbitol concentrations were decreased and delayed in both lentil cultivars after
7 days of treatment.
Heat stress inhibited chlorophyll synthesis in both Jordan 1 and Jordan 2 cultivars.
The Jordan 2 cultivar showed lower inhibition in chlorophyll synthesis under cold and heat stress than the Jordan 1 cultivar.
The chl a and chl b contents of both cultivars decreased as the NaCl concentration increased.
Mannitol treatments caused higher reduction in the chlorophyll contents in Jordan 2 seedlings than Jordan 1 seedlings.
The total chlorophyll contents for both lentil cultivars were reduced under all sorbitol treatments.
The results of this study indicated a direct effect of cold, heat, salt and osmotic abiotic stresses on seed viability and photosynthetic pigments in both lentil (Lens culinaris Medik) cultivars that are grown in Jordan.
Authors
N.A. Al-Quraan, M. Al-Sharbati, Y. Dababneh , M. Al-Olabi
Keywords
temperature stress, salt and osmotic stress, chlorophyll, Lens culinaris Medik, seed germination
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