Articles
EFFECT OF CARBON SOURCE ON PROTOCORM-LIKE BODY INDUCTION, PROLIFERATION AND REGENERATION IN DENDROBIUM SNOWFLAKE ‘RED STAR’
Article number
1078_15
Pages
113 – 120
Language
English
Abstract
An efficient micropropagation procedure is established for a nobile Dendrobium hybrid, D. Snowflake Red Star, by induction of protocorm-like bodies (PLBs). PLBs could be induced from shoot tips of axillary buds on Hyponex-based medium and the greatest induction rate occurred on medium supplemented with 0.03 M sucrose.
Among six types of carbon source tested, sucrose, glucose, fructose and maltose were better than sorbitol and mannitol for PLBs proliferation.
For PLB proliferation, in terms of fresh weight increase, sucrose at 0.06-0.09 M and maltose at 0.06-0.12 M were better than other concentrations.
For conversion of PLBs into shoots, maltose, sucrose, sorbitol, glucose and fructose showed number and fresh weight of regenerated shoot better than mannitol.
In addition, the number of regenerated shoot was better on medium supplemented with maltose at 0.03-0.12 M.
Among six types of carbon source tested, sucrose, glucose, fructose and maltose were better than sorbitol and mannitol for PLBs proliferation.
For PLB proliferation, in terms of fresh weight increase, sucrose at 0.06-0.09 M and maltose at 0.06-0.12 M were better than other concentrations.
For conversion of PLBs into shoots, maltose, sucrose, sorbitol, glucose and fructose showed number and fresh weight of regenerated shoot better than mannitol.
In addition, the number of regenerated shoot was better on medium supplemented with maltose at 0.03-0.12 M.
Publication
Authors
W. Udomdee, P.J. Wen, S.W. Chin, F.C. Chen
Keywords
nobile Dendrobium, PLB, sucrose, carbon source
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