Articles
SHORT-TERM STORAGE OF ALGINATE-ENCAPSULATED PROTOCORM-LIKE BODIES OF PHALAENOPSIS CORNU-CERVI (BREDA) BLUME & RCHB. F.
Article number
1078_9
Pages
75 – 80
Language
English
Abstract
Protocorm-like bodies (PLB) of Phalaenopsis cornu-cervi (Breda) Blume & Rchb. f. derived from wounded protocorm segments culturing on ½ Murashige and Skoog, 1962 (MS) medium containing 0.1 mg/L NAA and 0.1 mg/L TDZ. Encapsulation of the PLB using 3% sodium alginate and 100 mM calcium chloride dissolved in ND liquid medium supplemented 2% (w/v) sucrose.
Among the three temperature regimes of storage (4±1°C, 8±1°C, 25±1°C), encapsulated PLB stored at 25±1°C, for 30 days showed maximum percentage of germination at 100 when culture on ND medium supplemented with 4% (w/v) sucrose with 0.2% (w/v) activated charcoal (AC). Plantlets regenerated from capsules were acclimatized successfully with 100% survival rate.
Morphological characteristics of those plantlets were normal and ploidy level was the same as controlled plantlets as revealed by flow cytometry.
Among the three temperature regimes of storage (4±1°C, 8±1°C, 25±1°C), encapsulated PLB stored at 25±1°C, for 30 days showed maximum percentage of germination at 100 when culture on ND medium supplemented with 4% (w/v) sucrose with 0.2% (w/v) activated charcoal (AC). Plantlets regenerated from capsules were acclimatized successfully with 100% survival rate.
Morphological characteristics of those plantlets were normal and ploidy level was the same as controlled plantlets as revealed by flow cytometry.
Publication
Authors
S. Rittirat, S. Klaocheed, K. Thammasiri
Keywords
wounded protocorm, plantlets
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