Articles

INVESTIGATION OF THE INTERACTION OF ENDOPHYTES AND POPLAR PLANTS IN IN VITRO CULTURE AND FIELD TRIALS

Article number
1099_52
Pages
439 – 442
Language
English
Abstract
In 2007 the European Union has announced the “2020 directive” for the promotion of renewable energy resources and sustainable agricultural processes with a focus on biofuels.
According to this directive in 2020 20% of energy is achieved by renewable resources, with agricultural processes being a large proportion.
Poplar is an important energy crop in Europe.
Growth and biomass are the primary parameters for studies in this system, with the aim to enhance the suitability of such commercial crops as renewable energy resources.
The growth-promoting interaction of bacteria and the plant are of special interest.
Colonization of endophytic bacteria in internal plant tissue can promote plant growth and stress resistance, and can enhance control of pathogens.
In vitro grown explants of the poplar hybrid (♀[Populus alba × (P. davidiana + P. simonii) × P. tomentosa]) free from culturable bacteria show significant differences in growth, root development and metabolic signature when compared to shoots inoculated with Paenibacillus sp. strain P22. We investigated the influence of this endophytic Paenibacillus isolate on the metabolism of poplar shoot explants, free from culturable endophytic bacteria, with gas chromatography coupled to time-of-flight mass spectrometry (GC-TOF-MS). Metabolite profiling of Paenibacillus-inoculated and non-inoculated poplar plants revealed a significant metabolic signature in the plant as a response to the presence of the bacteria.

Publication
Authors
A.M. Hanak, L. Fragner, B. Kopp, C. Wawrosch, F. Gössnitzer, W. Wanek, D. Ewald, K. Ulrich, W. Weckwerth
Keywords
Populus, Paenibacillus, energy crop, renewable resources, mass spectrometry
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