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Articles

RAD2seq: an efficient protocol for plant genotyping by sequencing

Article number
1147_1
Pages
1 – 8
Language
English
Abstract
Advances in next-generation sequencing (NGS), through multiplexed sequencing of barcoded samples in a single run, have driven the costs down to the point that is now feasible the re-sequencing of an entire mapping population, also in medium-sized genome species.
RAD-seq approach is becoming more popular since implementations of the original protocol have been proposed.
A RAD-seq protocol, based on a double-digestion, was recently developed, which reduces sample preparation costs by eliminating random shearing, while increasing the multiplexing power.
Here we present a modified two-enzymes RAD-seq (namely RAD2seq) protocol, that includes a biotin/streptavidin guided capturing step which increases the recovering rate of selected fragments, while reducing the cost and time of library preparation.

Publication
IX International Symposium on Artichoke, Cardoon and Their Wild Relatives
Authors
A. Acquadro, L. Barchi, E. Portis, N. Carrasquilla-Garcia, M. Tirone, S. Lanteri, C. Comino
Keywords
RADseq, GBS, next generation sequencing
Full text
https://doi.org/10.17660/ActaHortic.2016.1147.1
Online Articles (61)
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