Articles
Characterization of the plastid terminal oxidase gene in carrot-involvement in carotenoids accumulation during storage root development
Article number
1153_13
Pages
85 – 92
Language
English
Abstract
The plastid terminal oxidase (PTOX) gene is known to be implicated in the carotenoid biosynthesis pathway.
In the present work we characterize the Daucus carota L. PTOX gene (DcPTOX) and describe the relation between DcPTOX transcript level and carotenoids accumulation during storage root development of white, yellow, orange, red, and purple carrot cultivars.
Transcript analysis of five genes (PSY2, PDS, ZDS, LCYB, LCYE) known as being directly involved in carotenoid biosynthesis pathways was also performed.
PTOX sequence analysis reveals the presence of typical structural features of PTOXs from higher plants: conserved iron binding residues, conserved exon 8 amino acids and 6 conserved cysteins at the C-terminal region, and the presence of a chloroplast transit peptide at the N-terminus.
Expression analysis revealed the presence of DcPTOX transcripts in all cultivars, and an increase of transcripts during the time course of the experiment, with differential expression among cultivars in early stages of root growth.
Our results demonstrated that DcPTOX has similar profile to that of other carotenoid biosynthetic genes with high correlation to all.
In the present work we characterize the Daucus carota L. PTOX gene (DcPTOX) and describe the relation between DcPTOX transcript level and carotenoids accumulation during storage root development of white, yellow, orange, red, and purple carrot cultivars.
Transcript analysis of five genes (PSY2, PDS, ZDS, LCYB, LCYE) known as being directly involved in carotenoid biosynthesis pathways was also performed.
PTOX sequence analysis reveals the presence of typical structural features of PTOXs from higher plants: conserved iron binding residues, conserved exon 8 amino acids and 6 conserved cysteins at the C-terminal region, and the presence of a chloroplast transit peptide at the N-terminus.
Expression analysis revealed the presence of DcPTOX transcripts in all cultivars, and an increase of transcripts during the time course of the experiment, with differential expression among cultivars in early stages of root growth.
Our results demonstrated that DcPTOX has similar profile to that of other carotenoid biosynthetic genes with high correlation to all.
Authors
M.D. Campos, H.G. Cardoso, A. Nogales, C. Campos, B. Arnholdt-Schmitt
Keywords
DcPTOX, Daucus carota L., gene characterization, expression analysis, carotenoid biosynthesis
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