Articles
In vitro multiplication and rooting of two Czech sweet cherry cultivars
Article number
1161_49
Pages
303 – 308
Language
English
Abstract
The goal of this study was to identify factors affecting in vitro shoot and root production of two Czech sweet cherry (Prunus avium) cultivars, ‘Amid’ and ‘Kares Frühe’ under in vitro culture conditions.
This information is required prior to experiments with chemotherapy as a method of sanitation from viruses.
The two genotypes were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution.
The overall rate of contamination was 16.7%. Six proliferation MS media containing 1, 2 and 4 mg L-1 BAP (6-benzylaminopurine), 0.5 and 1 mg L-1 TDZ (thidiazuron) or 10 mg L-1 2iP (6-(g,g-dimethylallylamino) purine) were tested for effects on proliferation, callus formation and shoot morphology.
Multiplication rate values were relatively low and varied between 1.1 and 2.1. BAP at 4 mg L-1 was found to be more effective than TDZ and 2iP for shoot multiplication.
Rooting was promoted on MS medium supplied with 1 mg L-1 NAA (naphthaleneacetic acid). Root initiation started within 2 weeks.
The root induction was relatively low, 45% for ‘Kares Frühe’ and 28% for ‘Amid’. In conclusion, multiplication and rooting rates were sufficient for in vitro culture establishment, short-term maintenance and in vitro chemotherapy.
However, these relatively low rates are not satisfactory for in vitro production of plants on a commercial scale.
This information is required prior to experiments with chemotherapy as a method of sanitation from viruses.
The two genotypes were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution.
The overall rate of contamination was 16.7%. Six proliferation MS media containing 1, 2 and 4 mg L-1 BAP (6-benzylaminopurine), 0.5 and 1 mg L-1 TDZ (thidiazuron) or 10 mg L-1 2iP (6-(g,g-dimethylallylamino) purine) were tested for effects on proliferation, callus formation and shoot morphology.
Multiplication rate values were relatively low and varied between 1.1 and 2.1. BAP at 4 mg L-1 was found to be more effective than TDZ and 2iP for shoot multiplication.
Rooting was promoted on MS medium supplied with 1 mg L-1 NAA (naphthaleneacetic acid). Root initiation started within 2 weeks.
The root induction was relatively low, 45% for ‘Kares Frühe’ and 28% for ‘Amid’. In conclusion, multiplication and rooting rates were sufficient for in vitro culture establishment, short-term maintenance and in vitro chemotherapy.
However, these relatively low rates are not satisfactory for in vitro production of plants on a commercial scale.
Publication
Authors
J. Sedlak, F. Paprstein
Keywords
Prunus avium, micropropagation, shoot, roots, 6-benzylaminopurine, thidiazuron
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