Home > XIV EUCARPIA Symposium on Fruit Breeding and Genetics > Danish apple cultivars: genetic diversity, parentage and breeding potential

Articles

Danish apple cultivars: genetic diversity, parentage and breeding potential

Article number

1172_66

Pages

355 – 358

Language

English

Abstract

We made a genetic analysis of about 500 apple cultivars, which either originate from Denmark or have been of greater importance in Denmark.
To study the diversity and parentage among the cultivars, 15 SSR markers were used.
A new PCR-based protocol for identification of S-alleles was developed and used to study the S-alleles among cultivars.
The protocol using general S-locus primers and digestion with two restriction enzymes, for fragment analysis on an automated DNA sequencer fastens the process and gives an accurate estimation of fragment lengths.
The ploidy level was determined using flow-cytometry.
All cultivars were distinguishable by the SSR-markers though Danish originating cultivars did not form a group distinct from foreign cultivars.
The parentage of several well-known cultivars was revealed.

Publication

XIV EUCARPIA Symposium on Fruit Breeding and Genetics

Authors

B. Larsen, C. Pedersen, M. Ørgaard, T. Toldam-Andersen

Keywords

genetic characterization, microsatellite markers, S-alleles, gene bank, Malus domestica

Full text

https://doi.org/10.17660/ActaHortic.2017.1172.66

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