Articles
‘Omic’ profiling of B. oleracea challenged with Xanthomonas campestris pv. campestris
Article number
1202_9
Pages
63 – 68
Language
English
Abstract
‘Omic’ approaches have emerged as valuable technologies to monitoring the biological status of an organism.
Among all their applications, the study of plant-pathogen interactions is on the cutting edge of plant research.
Regarding to Brassica crops diseases, the causal agent of black rot (Xanthomonas campestris pv. campestris, Xcc) is one of the most devastating soilborne diseases worldwide.
However, despite the black rot impact, few molecular and biochemical mechanisms of Brassica responses against Xcc have been elucidated.
In this work, we investigate molecular changes produced on Brassica oleracea plants infected by Xcc using different ‘omic’ technologies.
We carried out transcriptomic (MACE), proteomic (Maldi-TOF-TOF) and metabolomic (UHPLC-QTOF) analyses from leaves collected at 3 and 12 days post-infection.
In all cases, infection causes deep changes on Brassica metabolism and these changes are different between the early and late response.
Transcriptomics and proteomics results mainly showed an up-regulation of gene transcription and translation whereas metabolomic analysis indicated a down-regulation of most of the differentially expressed metabolites.
In general, processes related with primary and energetic metabolism are repressed whereas those related with resistance and signaling are up-regulated.
In conclusion, this study represents a step forward the understanding of B. oleracea-Xcc interaction and gives a good picture of the status of B. oleracea challenged with Xcc.
Among all their applications, the study of plant-pathogen interactions is on the cutting edge of plant research.
Regarding to Brassica crops diseases, the causal agent of black rot (Xanthomonas campestris pv. campestris, Xcc) is one of the most devastating soilborne diseases worldwide.
However, despite the black rot impact, few molecular and biochemical mechanisms of Brassica responses against Xcc have been elucidated.
In this work, we investigate molecular changes produced on Brassica oleracea plants infected by Xcc using different ‘omic’ technologies.
We carried out transcriptomic (MACE), proteomic (Maldi-TOF-TOF) and metabolomic (UHPLC-QTOF) analyses from leaves collected at 3 and 12 days post-infection.
In all cases, infection causes deep changes on Brassica metabolism and these changes are different between the early and late response.
Transcriptomics and proteomics results mainly showed an up-regulation of gene transcription and translation whereas metabolomic analysis indicated a down-regulation of most of the differentially expressed metabolites.
In general, processes related with primary and energetic metabolism are repressed whereas those related with resistance and signaling are up-regulated.
In conclusion, this study represents a step forward the understanding of B. oleracea-Xcc interaction and gives a good picture of the status of B. oleracea challenged with Xcc.
Publication
Authors
M. Tortosa, M.E. Cartea, V.M. Rodríguez, P. Velasco
Keywords
biotic stress, Xanthomonas campestris pv. campestris, omics
Online Articles (24)