Articles
Evaluation of genetic diversity within sweet cherry accessions of Czech genetic resources by molecular SSR markers
Article number
1235_9
Pages
71 – 78
Language
English
Abstract
Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history.
Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability.
In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of individual accessions, so that we eliminated duplications and characterized genetic relationships.
Recently, there are many different molecular methods but the most reliable are SSR markers.
In our work, we used PCR primer combinations for 20 SSR and 2 EST-SSR markers for analyses of 82 current, old and local sweet cherry cultivars from Czech genetic resources of the Research and Breeding Institute of Pomology in Holovousy.
In total, 113 polymorphic markers were amplified which we used for hierarchical cluster analysis of genetic variability.
The resulting dendrogram corresponded with genealogical and geobotanical characteristics of individual accessions which were divided into five clusters.
The breeding history of several known accessions was also proved.
Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability.
In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of individual accessions, so that we eliminated duplications and characterized genetic relationships.
Recently, there are many different molecular methods but the most reliable are SSR markers.
In our work, we used PCR primer combinations for 20 SSR and 2 EST-SSR markers for analyses of 82 current, old and local sweet cherry cultivars from Czech genetic resources of the Research and Breeding Institute of Pomology in Holovousy.
In total, 113 polymorphic markers were amplified which we used for hierarchical cluster analysis of genetic variability.
The resulting dendrogram corresponded with genealogical and geobotanical characteristics of individual accessions which were divided into five clusters.
The breeding history of several known accessions was also proved.
Publication
Authors
J. Patzak, A. Henychová, F. Paprštein, J. Sedlák
Keywords
sweet cherry (Prunus avium L.), SSR and EST-SSR markers, genetic diversity, genetic resources
Groups involved
Online Articles (70)