Articles
Cherry senescence – a response to application of ABA and partial sequence alignment of α-mannosidase gene
Article number
1275_54
Pages
391 – 398
Language
English
Abstract
This research was conducted to follow physiological characteristics of senescence in two cherry cultivars, an early and a late ripening cultivar, Karaj and Takdaneh, respectively.
The effect of ABA (0 and 200 ppm) on fruits of these two cultivars, Karaj and Takdaneh, within a period of 20 days after harvest and every 5 days, was evaluated.
Firmness reduced as storage period increased for both ABA and control treatment.
However, the rate of firmness reduction within ABA treatment was significant.
Gene of α-mannosidase was being traced up in five cherry cultivars including Early cultivar (ER), Foreign Early cultivar (FE), Ghazvin Early (GE), Mid ripe cultivar (MR) and Late cultivar (LR). Three primers were designed based on the gene sequence of peach extracted from NCBI. In this respect, the DNAs were extracted from leaves of the samples.
The primers could cover to 3100 bp length of the gene.
Based on the data sequence and application of Clustal X and 3 View software programs, the dendogram was drawn and similarity matrix was calculated.
The results revealed more similarity among ER, FE and LR rather than GH, GE and MR. Finally, GR and MR consisted two separate sub cluster of the main cluster.
The effect of ABA (0 and 200 ppm) on fruits of these two cultivars, Karaj and Takdaneh, within a period of 20 days after harvest and every 5 days, was evaluated.
Firmness reduced as storage period increased for both ABA and control treatment.
However, the rate of firmness reduction within ABA treatment was significant.
Gene of α-mannosidase was being traced up in five cherry cultivars including Early cultivar (ER), Foreign Early cultivar (FE), Ghazvin Early (GE), Mid ripe cultivar (MR) and Late cultivar (LR). Three primers were designed based on the gene sequence of peach extracted from NCBI. In this respect, the DNAs were extracted from leaves of the samples.
The primers could cover to 3100 bp length of the gene.
Based on the data sequence and application of Clustal X and 3 View software programs, the dendogram was drawn and similarity matrix was calculated.
The results revealed more similarity among ER, FE and LR rather than GH, GE and MR. Finally, GR and MR consisted two separate sub cluster of the main cluster.
Authors
B. Rahmani, M. Sharifani, S. Navabpour, F. Varasteh
Keywords
cherry, senescence, early and late cultivars, α-mannosidase gene
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