Home > I International Symposium on Botanical Gardens and Landscapes > Competent callus induction from shoot segments of Sangmon ‘Nuan Rajinee’ bamboo (Dendrocalamus sericeus Munro)

Articles

Competent callus induction from shoot segments of Sangmon ‘Nuan Rajinee’ bamboo (Dendrocalamus sericeus Munro)

Article number

1298_40

Pages

293 – 298

Language

English

Abstract

Callus was induced from 1 cm long of Sangmon ‘Nuan Rajinee’ bamboo; subsequently shoot multiplication was produced from callus using MS medium supplemented with or without 2,4-D, BA, NAA, and kinetin.
Our results indicated that there was no callus formation on MS medium without plant growth regulators.
However, when BA at 1.0 mg L^‑1 or kinetin at 2.0 mg L^‑1 in combination with 2,4-D at 3.0 mg L^‑1 were added and supplemented with or without casein hydrolysate, L-proline, and L-glutamine at a concentration of 500 mg L^‑1, 73.3-86.6% callus was found in all media.
Afterwards, callus was transferred to shoot induction medium.
The results showed that MS media supplemented with 1.0 mg L^‑1 BA and 0.1 mg L^‑1 TDZ gave the highest number of shoots at 12.4 shoots, significantly different from number of shoots obtained from other treatments.

Publication

I International Symposium on Botanical Gardens and Landscapes

Authors

P. Karawak, K. Obsuwan, S. Sengsai, C. Thepsithar

Keywords

micropropagation, multiple shoots, auxin, cytokinin, proline, glutamine, casein hydrolysate

Full text

https://doi.org/10.17660/ActaHortic.2020.1298.40

Groups involved

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