Home > I International Symposium on Botanical Gardens and Landscapes > Effects of BA and TDZ for in vitro shoot multiplication of three Hedychium species

Articles

Effects of BA and TDZ for in vitro shoot multiplication of three Hedychium species

Article number

1298_49

Pages

353 – 358

Language

English

Abstract

Hedychium is a terrestrial zingiberaceous species with underground rhizomes, as a source of raw materials for pharmaceutical and cosmetic products.
Therefore, demand for raw plant material has increased, resulting in overcollection from the wild.
In this study, two plant growth regulators (PGRs) were used for in vitro shoot multification of three Hedychium species.
The sterilized young shoot tips of H. stenopetalum, H. neocarneum, and H. flavescens were cultured on Murashige and Skoog (MS, 1962) supplemented with various concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ). All cultures were incubated under 16 h illiumination day^‑1 at 25±2°C for 8 weeks.
The results showed that the medium supplemented with 2.7 µM TDZ gave the highest shoot multiplication of H. flavescens and H. stenopetalum at 5.7 and 5.9 shoots explant^‑1, respectively.
The medium supplemented with 0.9 µM TDZ gave the highest multiplication of 5 shoots explant^‑1 in H. neocarneum. This significant study could be applied for future large-scale production.

Publication

I International Symposium on Botanical Gardens and Landscapes

Authors

P. Klaharn, P. Pumisutapon, K. Songnun, S. Rodpradit

Keywords

Zingiberaceae, micropropagation, plant growth regulator, cytokinin

Full text

https://doi.org/10.17660/ActaHortic.2020.1298.49

Groups involved

Online Articles (91)

1. Botanical garden and plant diversity conservation in China

S.J. Pei | K. Thammasiri | Z.W. Yang

2. Water and nutrient management in botanical gardens and horticulture

U. Schindler | F. Eulenstein

3. Development of the collection of floral-ornamental plants of the Nikitsky Botanical Gardens

4. The Nikitsky Botanical Gardens is the center of crop breeding development and biodiversity conservation

Yu.V. Plugatar

5. Development of a master plan and a fund-raising plan for the M.S. Swaminathan Botanical Garden in Wayanad, India

S. Krishnan | A. Kumar | M. Swaminathan

6. The natural and cultural characteristics of the Chinese Botanical Garden Exhibition System

7. People pollinating partnerships: harnessing collaborations between botanic gardens and agricultural research organizations on crop diversity

8. Exposition collection complex of spring-blooming plants presented in the Nikita Botanical Gardens

I.I. Golovnev | L.D. Komar-Tyomnaya | O.I. Korotkov | E.E. Golovneva

9. The University of Bologna Botanical Gardens: proposal for a tech-savvy walk throughout history

A. Minelli | E. Felice | I. Pasini | M.E. Giorgioni | F. Orsini

10. From microgarden technologies to vertical farms: innovative growing solutions for multifunctional urban agriculture

11. A taxonomic key for identifying seeds of undesirable landscape plants

P.S. Allen | S.C. Mogensen

12. Residential garden as green infrastructure: a research-by-design case study in Chiang Mai

N. Chareonlertthanakit | E. Yaipimol | V. Surinseng | C. Wanitchayapaisit | P. Suppakittpaisarn

13. University-trained volunteers use demonstration gardens as tools for effective and transformative community education

K. Reid | M. Gable

14. Design of fire-affected school grounds of special primary and secondary schools

A.T. Paraskevopoulou | K. Aggelakopoulos | E. Stathopoulou

15. Bridging interdisciplinary knowledge for sustainable urban landscapes: results from the international student competition UrbanFarm2019

16. Floral visitors of Kluai Bua Si Som (Musa rubra – Musaceae): an ornamental plant in Thailand

W. Nilapaka | T. Jenjittikul | A. Stewart | K. Tedsungnoen | S. Chomchalow Swangpol

17. Identifying obstacles for autonomous mowers in the United States landscape

A.J. Brown | P.S. Allen

18. Reflecting properties of ornamental plants for directing traffic in public park

S. Soontornyatara | S. Phanchai | K. Khaobang

19. Design of an aquaponic system for integrated fish and plant production in Bologna (Italy)

L. Settanni | F. Lombardo | G.M. Tamborra | F. Orsini

20. PINECUBE: technologies for sustainable plant production and urban renewal in Belluno (Italy)

21. Traditions and actual state of flower-ornamental crops’ use in landscape design in southern Russia

22. Aquagene: a unique conservatory for aquatic plants of India at KSCSTE – MBGIPS, Kerala, India

N.S. Pradeep | K.P. Anoop | P.V. Madhusoodhanan | S. Pradeep Kumar

23. Cryopreservation of Fragaria × ananassa using different techniques

S. Gupta | P. Tewari

24. Clematis plants conservation under in vitro genebank conditions

I.V. Mitrofanova | N.N. Ivanova | V.A. Brailko

25. Structural and functional changes in some species of Lamiaceae family in the process of in vitro genebank development

V.A. Brailko | I.V. Mitrofanova | I.V. Zhdanova | N.N. Ivanova

26. Pollen viability and the preliminary study of pollen preservation method of Amorphophallus koratensis Gagnep. (Araceae), the potential exotic ornamental plant

S. Soonthornkalump | U. Meesawat | P. Sutthinon

27. Conservation of Dendrobium cruentum Rchb. f.

28. Cryopreservation of Paphiopedilum exul (Ridl.) Rolfe seeds using encapsulation-vitrification and encapsulation-dehydration methods

T. Imsomboon | K. Thammasiri

29. Conservation and clonal micropropagation of rare species Gladiolus palustris Gaudin

O.I. Molkanova | Yu.N. Gorbunov | I.V. Shirnina

30. Cryopreservation of Dendrobium draconis Rchb. f. protocorms using V cryo-plate method

N. Jitsopakul | P. Homchan | K. Thammasiri

31. Current status of DOA Genebank Thailand in plant genetic resource conservation and utilization in the year 2019

32. Biotechnology strategy of plant biodiversity conservation in botanical gardens of Russia

I.V. Mitrofanova | O.I. Molkanova

33. Shoot multiplication of ‘HQC34’ hybrid pineapple (Ananas comosus (L.) Merr.) via bioreactor with different concentrations of 6-benzylaminopurine and MS medium

L. Valentina | O. Sittichan | S. Sripaoraya

34. Establishment of a protocol for micropropagation of Clausena guillauminii Tanaka

S. Kanjanawattanawong | N. Singbumrung

35. In vitro morphogenesis in endangered plant Seseli lehmannii Degen

I.V. Mitrofanova | O.V. Mitrofanova | M.I. Rudenko | N.P. Lesnikova-Sedoshenko | T.N. Kuzmina

36. An efficient in vitro plantlet regeneration of an ornamental aquatic plant Anubias heterophylla through shoot tip culture

S. Rittirat | S. Klaocheed | K. Thammasiri | A. Walam

37. Effects of BA and NAA on shoot multiplication of Grammatophyllum speciosum Blume in vitro

S. Samala | S. Taweerodjanakarn | J. Thipwong | K. Thammasiri | P.P. Chareonsap

38. Effects of BA, NAA, and activated charcoal on micropropagation of Nepenthes mirabilis (Lour.) Druce

A. Sapaeing | P. Sutthinon | A. Hilae | N. Wattanapan

39. In vitro culture of Enhalus acoroides (L.F.) Royle

Y. Meksuwan | P. Sutthinon | R.J. Ritchie

40. Competent callus induction from shoot segments of Sangmon ‘Nuan Rajinee’ bamboo (Dendrocalamus sericeus Munro)

P. Karawak | K. Obsuwan | S. Sengsai | C. Thepsithar

41. Enhancement of adenine sulfate and growth promoting substances on multiple shoot induction of Sangmon ‘Nuan Rajinee’ bamboo (Dendrocalamus sericeus Munro)

P. Karawak | K. Obsuwan | S. Sengsai | C. Thepsithar

42. Shoot initiation and multiple shoot induction of Sangmon ‘Nuan Rajinee’ bamboo (Dendrocalamus sericeus Munro)

W. Sareeyuvaratch | S. Sengsai | K. Obsuwan | C. Thepsithar

43. Effects of some organic additives on shoot initiation and shoot multiplication of Sangmon ‘Nuan Rajinee’ bamboo (Dendrocalamus sericeus Munro)

C. Koolprueksee | S. Sengsai | K. Obsuwan | C. Thepsithar

44. In vitro propagation and callus induction of Hedychium longicornutum Griff. ex Baker using different explants

R. Chuengpanya | N. Chuenboonngarm | K. Sakchaichanchol | A. Muangkroot | K. Thammasiri

45. In vitro propagation of Juniperus phoenicea L.

K. Bertsouklis | A.T. Paraskevopoulou | N. Zarkadoula

46. Investigation of micropropagation of the Mediterranean xerophyte Thymelaea hirsuta (L.) Endl. (Thymelaeaceae)

A.N. Martini | M. Papafotiou

47. Effect of plant growth regulators and agar concentration on shoot multiplication and hyperhydricity of Anthyllis barba-jovis

G. Vlachou | M. Trigka | M. Papafotiou

48. In vitro culture of mandarin orange (Citrus reticulate (L.) Blanco) via organogenesis

K. Obsuwan | C. Thepsithar | C. Chom-ngam

49. Effects of BA and TDZ for in vitro shoot multiplication of three Hedychium species

P. Klaharn | P. Pumisutapon | K. Songnun | S. Rodpradit

50. Phytochemical content and antioxidant activity of leaf extracts from nine bamboo species and determination of flavone C-glycosides by TLC and HPLC

P. Karawak | S. Sengsai | C. Thepsithar | S. Maksup

51. Anticancer efficacy of Luisia zeylanica Lindl., an epiphytic orchid from Eastern Ghats of Andhra Pradesh, India – an in vitro approach

S.M. Khasim | K. Jhansi | R. Venkatesh

52. Asexual and sexual propagation of Muntingia calabura L.

F. Nasution | C. Yapwattanaphun | J. Chumpookam

53. Vapour phase antifungal activity of essential oils and their raw materials against Aspergillus sp., Curvularia lunata, and Fusarium sacchari

A. Ahebwa | R. Mongkol | M. Kanjanamaneesathian

54. Efficacy of ethanolic extracts of galangal (Alpinia galanga) in inhibiting the mycelial growth of Fusarium sacchari and Curvularia lunata and their phytotoxicity on pak choi (Brassica rapa var. chinensis)

M. Molika | M. Kanjanamaneesathian | R. Mongkol

55. Improve growth, yield and quality of Jerusalem artichoke tuber by controlled spectral distribution lighting with LED

T. Seedapalee | P. Hongpakdee

56. Site analysis and development of strategy to enhance the identity of Lycabettus Hill in Athens and support sustainable management

57. Effect of substrate type and cultivation position on growth and safety to consume of the edible medicinal species Crithmum maritimum L., in an extensive urban green roof in Athens (Greece)

A.N. Martini | M. Papafotiou | Ι. Massas | N. Chorianopoulou | I. ¿ivanović

58. Effect of hydroponic and organic fertilizer on growth, safety, and storage quality of watercress (Nasturtium officinale)

S. Suvunnaphom | K. Sananon | M. Saouy | S. Kunyamee

59. Summer condition promoted growth, development, and carbohydrate content in sacred lotus (Nelumbo nucifera Gaertn.)

S. Suangto | S. Ruamrungsri | P. Hongpakdee

60. Controlled porosity based sub-surface porous vessel (SSPV) structures for irrigating organic farms of fruits and vegetables

61. Effect of paclobutrazol on growth of young potted flame tree (Delonix regia (Hook.) Raf.)

P. Srikoat | A. Pichakum | P. Boonkorkaew | N. Pichakum

62. Acoustic diagnostics of phytopathogenic damage of the trunk wood of Magnolia grandiflora L.

V.N. Gerasimchuk | Yu.V. Plugatar

63. The effect of preventing transition from mild cognitive impairment to early dementia by hand care treatment in the environment of horticultural therapy garden

64. Comparative effectiveness of various protection systems of the pear from Psilla pyri L. in the Crimea

E. Balykina | D. Korzh | V. Gorina | L. Yagodinskaya

65. Physicochemical and prebiotic properties of champedak (Artocarpus integer Merr.) seed starch

S. Taweerodjanakarn | S. Samala | A. Juemanee

66. Effect of different combinations of NAA and TDZ for shoot induction in vitro culture of Aglaonema simplex (Blume) Blume

S. Soontornyatara | P. Klammorn

67. Effects of time and concentration of ethyl methane sulfonate (EMS) on chia (Salvia hispanica L.)

S. Thaboran | D. Ketthaisong | C. Lapjit

68. DolomiNet: building a network of vertical farms in the heart of Italian Alps

69. Evaluation of phenotyping and genotyping for resistance against Tomato yellow leaf curl Thailand virus (TYLCTHV) validated by Ty-2, Ty-3, and Ty-4 genes in tomato

K. Krupeu | S. Techawongstien | C. Lapjit

70. Flavonoid contents and antityrosinase activity in various parts of Dendrobium ‘Sonia Jo Daeng’ and Dendrobium ‘Khao Sanan’ at different growth stages

S. Maksup | K. Obsuwan

71. Influence of geographical and genetic variation on GABA content in Annona muricata grown in Thailand

72. The effect of salinity on Chrysanthemum ‘Amiko red’

A.T. Paraskevopoulou | Ε. Mollal-Halil | G. Liakopoulos | P. Londra | K. Bertsouklis

73. Effects of carnauba wax and temperatures during storage on antioxidants and granulation in ‘Manee-Esan’ pomelo

C. Choeichaiyaphum | R. Nampila | S. Techawongstien

74. Effect of light-emitting diodes (LEDs) on growth of sweet basil (Ocimum basilicum) in an aquaponics system

T. Paising | S. Techawongstien | C. Lapjit

75. Study on organic acid and anti-oxidant properties of Elaeagnus latifolia L.

C. Niwaspragrit | P. Ratanachamnong | M. Munyanont | R. Deewatthanawong

76. The results of studying the adaptive potential of the walnut

S. Khokhlov | V. Melnikov | E. Panyushkina

77. The quality of the DNA isolated from Lavandula angustifolia leaves

78. Growth of golden oregano on an urban extensive green roof as affected by substrate type and irrigation frequency

M. Papafotiou | Ι. Adami

79. Effect of substrate type and irrigation frequency on growth of the bee-friendly plant Thymus citriodorus on an urban extensive green roof

M. Papafotiou | Ι. Adami

80. The effects of seed coat scarification, priming, and cold stratification on seed germination of a tropical night-blooming waterlily hybrid Nymphaea rubra ‘Maeploi’

81. Comparative evaluation of rooting cuttings of five Mediterranean sage species (Salvia sp.) native to Greece

A.N. Martini | K. Bertsouklis | G. Vlachou | E. Dariotis | M. Papafotiou

82. Comparative evaluation of seed germination of five Mediterranean sage species (Salvia sp.) native to Greece

G. Vlachou | Α.Ν. Martini | Ε. Dariotis | Μ. Papafotiou

83. Influence of hyperthermal stress on the functional state of persimmon (Diospyros kaki Thunb.) leaf apparatus

S. Khokhlov | S. Tsiupka | V. Melnikov | V.A. Brailko

84. Device-supported spread of experimental results in a rose trial garden

M.E. Giorgioni | A. Minelli | E. Felice | F. Orsini

85. Chromatographical profiles and microscopical characteristics of four Callicarpa species leaf samples collected in Thailand

P. Sithisarn | S. Muensaen | S. Jarikasem

86. A comparison of flooding tolerance of guava tree propagated from shoot layering and seedling

S. Kongsri | P. Nartvaranant | U. Boonprakob

87. Morphological and physiological responses of Thai commercial pummelo cultivars under salt stress

S. Kongsri | P. Usuwan | P. Nartvaranant

88. The development of female gametophyte in hermaphrodite flowers of ‘Pluk Mai Lie’ papaya (Carica papaya L.)

P. Buathongjan | K. Thaipong | A. Krairod | U. Doungngern | P. Burns

89. Successes in distant hybridization of cereals

V. Upelniek | L. Ivanova | N. Kuznetsova | S. Zavgorodny

90. Expression of anthocyanin biosynthetic genes in ornamental bananas

P. Kongsawadworakul | P. Vattanatam | W. Inta | U. Viboonjun | S.C. Swangpol

91. Biologically active substances of aboveground mass of Artemisia salsoloides Willd.

O.M. Shevchuk | O.I. Korotkov | L.A. Logvinenko | E.V. Malaeva